Anti-TAK1 (MAP3K7) Monoclonal Antibody for WB, IP, ELISA - O43318

Core Product Specifications and Parameters

Biological Background: TAK1 Function and Localization

• TAK1 (MAP3K7) is a mitogen-activated protein kinase kinase kinase that serves as an essential component of the MAP kinase signal transduction pathway, mediating cellular responses to environmental changes. Related references: PMID:10094049 | PMID:11460167 | PMID:12589052
• It transduces signals from diverse stimuli including TRAF6, IL-1, TGF-beta, TLR, CD40, and BCR by acting as an upstream activator of the MKK/JNK and p38 MAPK cascades, leading to AP-1 transcription factor activation. Related references: PMID:16893890 | PMID:9079627 | PMID:11460167 | PMID:8663074
• Independently of MAP2Ks, TAK1 promotes NF-kappa-B activation by phosphorylating IKBKB in the IKK complex upon recruitment to polyubiquitin chains via TAB2/TAB3. Related references: PMID:12589052 | PMID:8663074
• TAK1 plays a major role in osmotic stress signaling by activating MAPK8/JNK1, but not NF-kappa-B. Related references: PMID:16893890
• The protein is localized in the cytoplasm and at the cell membrane, consistent with its role in signal transduction from receptors.
• Multiple isoforms exist with tissue-specific expression: isoform 1A abundant in ovary, skeletal muscle, spleen, and blood mononuclear cells; isoform 1B in brain, kidney, small intestine; isoform 1C in prostate; isoform 1D is less abundant.
• TAK1 is subject to various post-translational modifications including phosphorylation, acetylation, and ubiquitination, which regulate its activity and signaling specificity.
• Additionally, TAK1 has roles in antiviral restriction (TRIM5 capsid-specific restriction) and in necroptosis by phosphorylating RIPK1; it phosphorylates STING1 in response to cGAMP, promoting STING1 translocation to COPII vesicles. Related references: PMID:21512573 | PMID:37832545

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 507-606) of human TAK1; this region may be less conserved, potentially contributing to species cross-reactivity with mouse. For WB, a band around 67 kDa is expected; consider using appropriate loading controls and KO-validated lysates to confirm specificity.
• In IP experiments, pre-clear lysates and optimize antibody-to-lysate ratios; the rabbit monoclonal format provides high affinity and reproducibility.
• For ELISA, the antibody can be used for capture or detection; validate with recombinant TAK1 protein or cell lysates expressing the target.
• Note that cross-reactivity is reported for human and mouse; reactivity with other species should be verified experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, bridging research and diagnostic development. For TAK1 (MAP3K7), a central kinase in MAPK signaling and inflammation, we provide validated antibody pairs, recombinant monoclonal antibodies, and bulk ancillary reagents to support your assay development. Our expert team also assists with rare target sourcing, ensuring seamless integration from concept to clinic.