Anti-TAGLN2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P37802

Core Product Specifications and Parameters

Biological Background: TAGLN2 Function and Localization

• Transgelin-2 (TAGLN2), also referred to as SM22-alpha homolog or epididymis tissue protein Li 7e, is a human protein encoded by the TAGLN2 gene (Gene ID: 8407).
• According to UniProt (P37802), the protein is expressed in the epididymis at the protein level.
• The protein undergoes alternative splicing, giving rise to multiple isoforms.
• Post‑translational modifications annotated in UniProt include acetylation, phosphorylation, methylation, isopeptide bond formation, and ubiquitin conjugation, indicating diverse regulatory mechanisms.
• The molecular weight of the canonical isoform is 22,391 Da (UniProt), consistent with the observed 22 kDa band.
• Keywords from the entry highlight a 3D-structure, direct protein sequencing evidence, proteomics identification, and inclusion in the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-158); confirm that alternative splicing does not alter this region if working with specific isoforms.
• For Western blotting, the predicted molecular weight is ~22 kDa; use a 10–12% SDS‑PAGE gel and validate with a known positive control.
• In immunofluorescence/ICC, optimize permeabilization conditions as transgelin-2 is associated with the actin cytoskeleton.
• For ELISA, consider using the recombinant immunogen as a coating antigen to establish a standard curve and validate antibody specificity.
• Cross-reactivity is reported for human and mouse; when extending to other species, check sequence homology of the immunogen peptide to avoid unexpected results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your global one‑stop platform for IVD raw‑material sourcing, delivering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare‑target sourcing to diagnostic manufacturers and research laboratories. This anti‑TAGLN2 rabbit polyclonal antibody supports biomarker discovery and immunoassay development in cytoskeletal and tissue‑specific protein studies. Contact our team for custom or bulk orders and experience seamless procurement with expert technical support—accelerating your path from concept to clinic.