Anti-TAGLN Polyclonal Antibody for WB, IHC-P, ELISA - Q01995

Core Product Specifications and Parameters

Biological Background: TAGLN Function and Localization

• Transgelin (TAGLN), also known as SM22-alpha or WS3-10, is a 22–23 kDa actin-binding protein encoded by the TAGLN gene.
• It functions as an actin cross-linking and gelling protein, contributing to the stabilization and organization of the actin cytoskeleton.
• TAGLN is involved in calcium-dependent regulation of cell contractility and has been implicated in replicative senescence.
• As a smooth muscle protein, TAGLN is predominantly expressed in smooth muscle tissues, where it plays a role in maintaining vascular contractile properties.
• Subcellularly, TAGLN localizes to the cytoplasm, associating with the actin cytoskeleton.
• The protein undergoes post-translational modifications including acetylation, methylation, and phosphorylation, which may regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide derived from the central region (aa 100–201) of human TAGLN. This region may be conserved across species, aligning with the observed cross-reactivity with rat.
• For Western blot, a band at approximately 23 kDa is expected in human and rat samples. Consider using appropriate positive controls such as smooth muscle lysates.
• IHC-P applications: antigen retrieval conditions may need optimization. Smooth muscle-rich tissues (e.g., aorta, bladder) are recommended as positive controls.
• ELISA: the purified antibody can be used for direct or sandwich ELISA; optimal coating concentrations should be determined experimentally.
• Given the potential for post-translational modifications, verify antibody specificity in the relevant biological context through peptide competition or knockout models.

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