Anti-TAF5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15542

Core Product Specifications and Parameters

Biological Background: TAF5 Function and Localization

• TAF5, also known as TAFII100, is an 87 kDa subunit of the TFIID basal transcription factor complex, which comprises TBP and 13 TAFs (TAF1- TAF13). Related references: PMID:33795473, PMID:8758937, PMID:8942982

• TFIID plays a pivotal role in RNA Polymerase II-dependent transcription by recognizing core promoters via the TATA-box-binding protein TBP and orchestrating pre-initiation complex (PIC) assembly. Related references: PMID:33795473

• TAF5 is a structural and functional component of two distinct TFIID modules: TFIID-A (with TAF3 and TBP) and TFIID-B (with TAF8). Related references: PMID:33795473

• It directly mediates protein-protein contacts between TFIID and the general transcription factor TFIIF within the PIC, contributing to initiation complex stability. Related references: PMID:33795473

• The protein localizes exclusively to the nucleus, consistent with its role in transcription regulation.

• TAF5 contains multiple WD repeat motifs and undergoes alternative splicing; it is also annotated with a host-virus interaction keyword, suggesting potential involvement in viral transcription processes.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 656-800, a region expected to be accessible; consider using nuclear extracts for Western blot to enrich the target protein.
• The reported molecular weight is approximately 87 kDa; a band near this size should be observed in WB, but validate with appropriate controls.
• For IF/ICC, permeabilization is required to detect nuclear localization; antibody dilution and fixation conditions may need optimization for each cell type.
• Cross-reactivity with mouse and rat has been documented; verify the target sequence homology in these species before interpreting results.
• ELISA can be used for quantitative detection; ensure proper coating and blocking protocols to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement of premium IVD raw materials for transcription and gene regulation research, including validated antibody pairs, monoclonal and polyclonal options, and bulk ancillary reagents. For rare or specialized targets like TAF5, we leverage global sourcing networks to deliver consistent, high-quality products tailored to diagnostic and laboratory needs. Partner with CamelBio to bridge your concept-to-clinic journey with reliable reagent supply.