Anti-TAF1C Polyclonal Antibody for WB, ELISA - Q15572

Core Product Specifications and Parameters

Biological Background: TAF1C Function and Localization

• TAF1C, also known as TAFI110 or TAFI95, is a core subunit of the SL1/TIF-IB complex that drives RNA polymerase I transcription initiation.
• It facilitates pre-initiation complex (PIC) assembly at ribosomal DNA (rDNA) promoters by stabilizing the binding of nucleolar transcription factor 1/UBTF and excluding TBP-TFIID interactions.
• TAF1C recruits RNA polymerase I to the rRNA gene promoter through a direct interaction with RRN3.
• The protein is localized to the nucleolus, consistent with its role in rRNA synthesis.
• TAF1C is subject to alternative splicing and phosphorylation, as identified by direct protein sequencing and proteomics.
• It functions as a DNA-binding phosphoprotein and is classified under transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 70–320 of human TAF1C, so the antibody likely recognizes an internal epitope; consider using nuclear or nucleolar extracts for Western blot to enrich the target.
• For cross-reactivity with mouse and rat samples, align the immunogen sequence with orthologous proteins to assess conservation; empirical validation in these species is recommended.
• As TAF1C is a transcription factor with potentially low abundance, optimize lysis buffers, protease inhibitors, and detection sensitivity for reproducible results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement of high-quality IVD raw materials, including antibodies like this anti-TAF1C polyclonal antibody for RNA polymerase I and nucleolar research. Beyond our catalog, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your assay development. For rare or custom targets, our sourcing network delivers reliable solutions from early research to clinical application.