Anti-TAF15 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q92804

Core Product Specifications and Parameters

Biological Background: TAF15 Function and Localization

• TAF15, also known as TATA-binding protein-associated factor 2N (TAF2N), 68 kDa TATA-binding protein-associated factor (TAFII68), and RNA-binding protein 56 (RBP56), is a multifunctional RNA and ssDNA-binding protein.
• It is implicated in transcription initiation, potentially mediating promoter-specific interactions as part of the RNA polymerase II preinitiation complex.
• TAF15 binds single-stranded RNA containing the consensus sequence 5'-AGGUAA-3'. Related references: PMID:21256132
• The protein localizes to both the nucleus and the cytoplasm, suggesting dynamic shuttling or dual functional roles.
• TAF15 is ubiquitously expressed in all human fetal and adult tissues.
• Post-translational modifications include acetylation, ADP-ribosylation, phosphorylation, methylation, and ubiquitination, indicating complex regulatory control.
• It contains zinc-finger domains, contributing to its nucleic acid binding properties, and is involved in chromosomal rearrangements associated with cancer, acting as a proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen, comprising amino acids 1–280 of human TAF15, targets the N-terminal region conserved among TAF15 isoforms; this broadens isoform coverage in detection assays.
• For Western blot (WB), a predominant band at approximately 62 kDa corresponding to full-length TAF15 may be observed; include proper loading controls.
• In immunohistochemistry (paraffin) and immunofluorescence, consider antigen retrieval techniques and validate signal specificity using negative controls.
• For immunoprecipitation and ELISA applications, optimize antibody concentrations and confirm detection in your sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to high-quality IVD raw materials, including validated antibodies like this TAF15 polyclonal antibody for transcription and cancer research. Our supply chain offers bulk quantities of ancillary reagents and supports rare-target sourcing, streamlining your diagnostic development from concept to clinic.