Anti-TAF12 Polyclonal Antibody for WB, ELISA - Q16514

Core Product Specifications and Parameters

Biological Background: TAF12 Function and Localization

• TAF12, also referred to as TAF2J, TAFII20, or TAFII15, is a core component of the general transcription factor TFIID, which orchestrates RNA polymerase II pre-initiation complex assembly at gene promoters. Related references: PMID:33795473
• In addition to TFIID, TAF12 is a shared subunit of the TATA-binding protein-free TAF complex (TFTC), the PCAF histone acetylase complex, and the STAGA transcription coactivator-HAT complex, linking transcription to chromatin acetylation. Related references: PMID:10373431 PMID:7729427 PMID:8598932
• TAF12 is localized to the nucleus, consistent with its role in transcription, and is expressed ubiquitously across human tissues.
• The protein is subject to multiple post-translational modifications, including phosphorylation and ubiquitin-like conjugation (e.g., isopeptide bond formation), which may modulate its activity or interactions.
• TAF12 has a calculated molecular weight of approximately 17.9 kDa, with an apparent mass of around 18 kDa on SDS-PAGE, and alternative initiation generates isoforms of varying lengths.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-161 of human TAF12; therefore, the antibody is expected to recognize epitopes within this N-terminal fragment.
• For Western blotting, a band at approximately 18 kDa is anticipated; use appropriate molecular weight markers and validate in a human sample with verified TAF12 expression.
• This polyclonal antibody may exhibit lot-to-lot variation; it is advisable to titrate the antibody for optimal performance in your specific assay conditions.
• For ELISA, direct or sandwich formats may be optimized using the recombinant immunogen as a standard.
• When applying to other species, verify cross-reactivity experimentally, as the antibody is validated only for human TAF12.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers with streamlined access to high-quality raw materials, including validated TAF12 antibodies like this product. Our catalog also features optimized antibody pairs, bulk ancillary reagents, and customized sourcing for rare transcription factor targets, supporting your assay development from concept to clinic.