Anti-TADA3 Polyclonal Antibody for WB, IF/ICC, ELISA - O75528

Core Product Specifications and Parameters

Biological Background: TADA3 Function and Localization

• TADA3 (Transcriptional adapter 3) is also known as ADA3, hADA3, STAF54, and TADA3L. It exists in multiple isoforms due to alternative splicing.
• As a core component of the PCAF complex, TADA3 facilitates histone acetylation in a nucleosomal context, analogous to the yeast SAGA complex, thereby regulating chromatin structure and gene expression.
• TADA3 additionally serves as a coactivator for p53/TP53-mediated transcriptional activation, linking it to tumor suppressor pathways.
• It is also a subunit of the ATAC complex, which catalyzes histone acetyltransferase activity specifically on histones H3 and H4.
• The protein localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modifications include acetylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation (Ubl), which may modulate its activity and interactions.
• TADA3 is ubiquitously expressed across human tissues, reflecting its fundamental role in cellular transcription.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 270–369) lies within a region that may be accessible; consider confirming specificity with knockdown or knockout controls.
• For western blot (WB), the expected molecular weight is approximately 49 kDa. Use appropriate loading controls and optimize antibody dilution for your sample.
• In immunofluorescence/immunocytochemistry (IF/ICC), TADA3 localizes to the nucleus; ensure adequate permeabilization and include DAPI counterstaining to confirm nuclear signal.
• For ELISA applications, validate the antibody’s binding to the recombinant immunogen or native protein under your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, CROs, and research labs to deliver reliable IVD raw materials, including validated antibodies and recombinant proteins for transcriptional research. Our portfolio features polyclonal and monoclonal antibodies optimized for assays like WB, IF/ICC, and ELISA, alongside bulk ancillary reagents. For rare targets like TADA3, we offer customized sourcing to meet your pipeline demands—bridging the gap from concept to clinical application.