Anti-TADA2B Polyclonal Antibody for WB, ELISA - Q86TJ2

Core Product Specifications and Parameters

Biological Background: TADA2B Function and Localization

• TADA2B (Transcriptional adapter 2-beta), also known as ADA2-beta, is a nuclear coactivator involved in transcription regulation.
• It coactivates PAX5-dependent transcription by interacting with either the SWI/SNF complex component SMARCA4 or the histone acetyltransferase GCN5L2 (KAT2A), suggesting a role in chromatin remodeling and histone acetylation.
• The protein contains zinc finger motifs (indicated by keywords: Zinc, Zinc-finger, Metal-binding), likely contributing to protein-protein or protein-DNA interactions.
• Alternative splicing generates multiple transcript variants (keyword: Alternative splicing), potentially producing isoforms with distinct regulatory functions.
• Subcellular localization is primarily nuclear, consistent with its role in transcriptional regulation.
• It is classified under the reference proteome and identified in high-throughput proteomics studies, underscoring its functional relevance in human biology.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is 48 kDa; consider using whole-cell lysates from human cell lines known to express TADA2B (e.g., HeLa, HEK293) as positive controls.
• As a rabbit polyclonal antibody targeting residues 150–322, it may recognize multiple isoforms; validate specificity in your model system by comparing with siRNA knockdown or knockout controls.
• In ELISA applications, direct binding assays or sandwich ELISA formats can be explored; titrate the antibody to determine optimal working concentrations.
• Non-specific binding can be minimized by optimizing blocking conditions (e.g., 5% BSA or non-fat milk in TBST) and including appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents. For transcription-related targets like TADA2B, we provide reliable sourcing solutions to accelerate your assay development. Contact our team for bulk orders or to explore custom material requirements.