Anti-TACR3 Rabbit Polyclonal Antibody for WB and ELISA - P29371

Core Product Specifications and Parameters

Biological Background: TACR3 Function and Localization

• TACR3 encodes the neuromedin-K receptor (NK-3R), also referred to as neurokinin B receptor, a G protein-coupled receptor (GPCR) that binds tachykinin peptide ligands.
• The receptor exhibits highest affinity for neuromedin-K (neurokinin B), with lower affinity for substance K and substance P (PubMed:1312036). Upon ligand binding, it triggers G(q)-mediated phospholipase C activation and phosphatidylinositol hydrolysis (PubMed:37391393). Related references: PMID:1312036, PMID:37391393
• Additionally, neuromedin-K binding promotes signaling through adenylate cyclase, leading to increased intracellular cAMP levels (By similarity).
• The receptor localizes to the cell membrane and belongs to the rhodopsin-like GPCR superfamily, characterized by seven transmembrane domains.
• Clinically, mutations in TACR3 are linked to congenital hypogonadotropic hypogonadism, a condition marked by deficient gonadotropin secretion and reproductive axis dysfunction.
• Post-translational modifications include N-linked glycosylation, palmitoylation at cysteine residues, and conserved disulfide bonds essential for receptor stabilization and trafficking.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular domain (aa 356–465), enabling recognition of both unmodified and post-translationally modified receptor forms; validate detection in your sample system.
• For Western blot, consider using a reducing agent and appropriate loading controls to confirm 52 kDa band specificity; a band shift may occur due to glycosylation.
• In ELISA applications, the recombinant immunogen can serve as a positive control; optimize coating conditions and antibody dilution for your specific assay format.
• Given cross-reactivity with mouse and rat, verify sequence homology in the immunogen region; if using these species, include species-specific positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive one-stop platform for IVD raw materials. This anti-TACR3 polyclonal antibody exemplifies our portfolio of GPCR-targeted reagents, supported by validated antibody pairs and optimized monoclonal/polyclonal antibodies. Beyond antibodies, we provide bulk ancillary reagents and can assist with sourcing rare or custom targets—ensuring you have the critical raw materials needed from concept to clinical application.