Anti-TACO1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9BSH4

Core Product Specifications and Parameters

Biological Background: TACO1 Function and Localization

• TACO1 (Translational Activator of Cytochrome c Oxidase 1), also known as CCDC44, is a mitochondrial protein that functions as a translational activator of mitochondrially-encoded cytochrome c oxidase I (MT-CO1).
• It contains a coiled-coil domain and is involved in the regulation of mitochondrial translation, specifically promoting the synthesis of COX1, a critical subunit of complex IV of the respiratory chain.
• Mutations in TACO1 are associated with Leigh syndrome, a severe primary mitochondrial disease characterized by neurological deterioration due to impaired oxidative phosphorylation.
• Subcellularly, TACO1 localizes to the mitochondrion, consistent with its role in mitochondrial gene expression.
• The protein has been identified in proteomics analyses as part of the reference proteome for humans.
• TACO1 is evolutionarily conserved and its loss leads to defective cytochrome c oxidase activity, highlighting its essential role in cellular respiration.

Experimental Guidance and Technical Tips

• For Western blot, expect a band at approximately 32 kDa; validate specificity using TACO1 knockout or knockdown cell lysates.
• Optimize antibody dilution for each application (WB, IHC-P, ELISA) with appropriate controls to account for lot-to-lot variation.
• For immunohistochemistry, consider antigen retrieval and confirm staining pattern using tissue sections known to express TACO1.
• Given the immunogen covers the N-terminal region (1-297), the antibody may detect full-length TACO1 and any N-terminal fragments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined pathway for IVD raw material procurement, including this anti-TACO1 polyclonal antibody tailored for mitochondrial disease and respiratory chain research. Our portfolio features validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Partner with us to accelerate your diagnostic development from concept to clinic.