Anti-TACC3 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y6A5

Core Product Specifications and Parameters

Biological Background: TACC3 Function and Localization

• TACC3 (Transforming acidic coiled-coil-containing protein 3), also known as ERIC-1, is a coiled-coil domain-containing protein identified through proteomics.
• Plays a critical role in centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors, coupling the nucleus and centrosome in a microtubule-dependent manner (By similarity).
• Acts as a component of the TACC3/ch-TOG/clathrin complex that stabilizes kinetochore fibers by forming inter-microtubule bridges, essential for maintaining kinetochore fiber tension during mitosis. Related references: PMID:21297582 PMID:23532825
• May be involved in the control of cell growth and differentiation; its dysregulation may contribute to cancer. Related references: PMID:14767476
• Localizes predominantly to the cytoplasm and cytoskeleton, specifically at the centrosome, mitotic spindle, and spindle poles.
• Subject to post-translational modifications including phosphorylation and acetylation, which regulate its function during cell division.
• Keywords associated include acetylation, cell cycle, cell division, mitosis, and phosphoprotein, reflecting its dynamic role in mitotic progression.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region of human TACC3, which is conserved across species; cross-reactivity with mouse and rat is supported.
• For Western blot, the predicted molecular weight is 90 kDa; consider using an appropriate gel percentage and optimized blocking conditions.
• In immunofluorescence/immunohistochemistry, permeabilization and antigen retrieval steps may be necessary; validate in the relevant sample system.
• Due to the role of TACC3 in mitosis, synchronizing cells or using cell cycle inhibitors could enrich mitotic populations for enhanced detection.
• As a rabbit monoclonal antibody, it generally offers high specificity and lot-to-lot consistency; nevertheless, it is advisable to validate performance in the intended application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For TACC3 research in cell cycle and cancer biology, we can source rare-target raw materials and provide technical support to streamline your development from concept to clinic.