Anti-TAB3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8N5C8

Core Product Specifications and Parameters

Biological Background: TAB3 Function and Localization

• TAB3 (also known as MAP3K7IP3, NAP1) is an essential adaptor protein required for the activation of JNK and NF-κB signaling pathways, playing a critical role in innate immune and inflammatory responses. Related references: PMID:14633987, PMID:14766965, PMID:15327770
• It functions as an adapter linking the kinase MAP3K7/TAK1 and the E3 ubiquitin ligase TRAF6 to Lys-63-linked polyubiquitin chains. Related references: PMID:14633987, PMID:14766965, PMID:15327770
• The RanBP2-type zinc finger (NZF) domain of TAB3 specifically recognizes Lys-63-linked polyubiquitin chains on substrate proteins such as RIPK1 and RIPK2, forming a signaling complex that promotes autophosphorylation of TAK1 and subsequent activation of the IKK complex. Related references: PMID:15327770, PMID:18079694, PMID:22158122
• TAB3 may act as an oncogenic factor, and its constitutive overexpression is observed in certain tumor tissues.
• Alternative splicing generates multiple transcript variants encoding different protein isoforms.
• TAB3 undergoes various post-translational modifications, including acetylation, methylation, phosphorylation, and ubiquitination (isopeptide bond formation).
• The protein contains a coiled-coil domain and a zinc-binding NZF domain, and is classified as a metal-binding phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 390–480 of human TAB3, covering a C-terminal region that may include functionally relevant domains; assess epitope accessibility in your specific assay format.
• For Western blot (WB), the predicted molecular weight of TAB3 is approximately 79 kDa; multiple bands corresponding to different isoforms or post-translational modifications may be observed; optimize blocking and antibody dilution.
• In immunohistochemistry (IHC-P), antigen retrieval and detection conditions may require optimization; consider using tissues with known TAB3 overexpression, such as tumor samples, as positive controls.
• For ELISA, consider pairing with a suitable detection antibody; the polyclonal nature may provide higher sensitivity but also requires careful background control.
• Since this is a polyclonal antibody, it recognizes multiple epitopes, which can enhance signal but may require validation for specificity in your target application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to supply high-quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our expertise in rare target sourcing and technical consultation supports the seamless integration of products like the TAB3 rabbit pAb into assays focused on NF-κB and JNK signaling, immunology, and oncology research. From concept to clinic, CamelBio is your trusted bridge for comprehensive raw-material solutions.