Anti-TAB3 Monoclonal Antibody for WB, ELISA - Q8N5C8

Core Product Specifications and Parameters

Biological Background: TAB3 Function and Localization

• TGF-beta-activated kinase 1 and MAP3K7-binding protein 3 (TAB3), also known as NF-kappa-B-activating protein 1 (NAP1) and TAK1-binding protein 3 (TAB-3), is an adapter protein required for the activation of JNK and NF-kappa-B signaling pathways. Related references: PMID:14633987 PMID:14766965 PMID:15327770
• It specifically recognizes Lys-63-linked polyubiquitin chains through its RanBP2-type zinc finger (NZF) domain, linking MAP3K7/TAK1 and TRAF6 to these chains. Related references: PMID:14633987 PMID:14766965 PMID:15327770
• The NZF domain recognizes polyubiquitin chains anchored to substrates like RIPK1 and RIPK2, scaffolding a signaling complex that promotes TAK1 autophosphorylation and subsequent activation of the IKK complex. Related references: PMID:15327770 PMID:18079694 PMID:22158122
• TAB3 is widely expressed and constitutively overexpressed in certain tumor tissues, suggesting a potential oncogenic role.
• The protein undergoes post-translational modifications including acetylation, methylation, and phosphorylation, and contains a coiled-coil domain, zinc-finger, and metal-binding sites.
• Alternative splicing generates multiple transcript variants, with a major and minor transcript reported.

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole-cell lysates from human or mouse cells, as the antibody detects a band around 79 kDa corresponding to TAB3.
• Due to the immunogen design (N-terminal peptide, aa 1-100), ensure the epitope is accessible in your assay; denaturing conditions for WB are appropriate.
• For ELISA, validate with recombinant TAB3 protein or peptide to assess binding affinity.
• The antibody cross-reacts with mouse TAB3, so it can be used for mouse model studies; verify expression levels in the relevant tissue.
• Consider including appropriate controls (e.g., TAB3 knockout lysates) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-material supply chain, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents tailored to your assay development needs. For researchers targeting the NF-kB and JNK signaling pathways, we offer consistent quality TAB3 antibodies and can source rare target raw materials to accelerate your diagnostic projects from concept to clinic.