Anti-TAB2 (MAP3K7IP2) Rabbit Polyclonal Antibody for WB, ELISA - Q9NYJ8

Core Product Specifications and Parameters

Biological Background: TAB2 (TGF-beta-activated kinase 1 and MAP3K7-binding protein 2) Function and Localization

• TAB2, also known as MAP3K7IP2 or TAB-2, is an adapter protein that links TRAF6 to MAP3K7/TAK1, enabling activation of JNK and NF-κB signaling cascades. Related references: PMID:10882101 PMID:11460167 PMID:15327770
• Its RanBP2-type zinc finger (NZF) domain specifically recognizes Lys-63-linked polyubiquitin chains, serving as a scaffold to promote MAP3K7/TAK1 autophosphorylation and subsequent IKK complex activation. Related references: PMID:15327770 PMID:18079694 PMID:22158122
• TAB2 also binds heterotypic Lys-63/Lys-48 branched ubiquitin chains, expanding its regulatory roles in Ub signaling. Related references: PMID:27746020
• It plays a critical role in heart development; in embryos it is expressed in ventricular trabeculae, endothelial cells of the outflow tract, and developing aortic valves. Related references: PMID:20493459
• Subcellularly, TAB2 localizes to membranes, endosomes, lysosomes, and the cytosol, consistent with its signaling scaffold functions.
• Post-translational modifications include phosphorylation and methylation; it is also subject to ubiquitin conjugation and isopeptide bond formation.
• Genetic variations in TAB2 are associated with chromosomal rearrangements and may contribute to disease phenotypes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 510–620 in the C‑terminal region of human TAB2; confirm reactivity with your specific sample system, especially when studying isoforms or diseased tissues.
• For Western blotting, the predicted molecular weight is ~76 kDa. Consider a starting dilution range of 1:500–1:2000 and include appropriate positive and negative controls.
• For ELISA applications, optimize coating antigen concentration and antibody dilution using standard checkboard titration.
• The antibody cross‑reacts with mouse and rat TAB2; verify performance in these species by running parallel controls.
• As a polyclonal reagent, lot‑to‑lot consistency should be monitored; we recommend including a reference standard in each experiment.

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