Anti-T1R3/TAS1R3 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q7RTX0

Core Product Specifications and Parameters

Biological Background: T1R3/TAS1R3 Function and Localization

• T1R3/TAS1R3 is a member of the taste receptor type 1 family, encoded by the TAS1R3 gene in humans. It functions as a subunit of heteromeric G protein-coupled receptor complexes.
• In combination with TAS1R1, it forms the umami taste receptor, responding to monosodium glutamate and other savory stimuli.
• With TAS1R2, it forms the sweet taste receptor, recognizing a wide range of natural and synthetic sweeteners, and is essential for trehalose detection.
• Sequence variations within and between species can significantly impact taste selectivity and sensitivity.
• The receptor is localized at the cell membrane, as a multi-pass transmembrane protein.
• T1R3/TAS1R3 undergoes glycosylation and contains a signal peptide, characteristic of cell surface receptors.
• It is involved in sensory transduction of taste, with broad relevance in food science, nutrition, and sensory biology.

Experimental Guidance and Technical Tips

• This antibody was developed using an immunogen corresponding to amino acids 400-570 of human T1R3/TAS1R3, covering a region within the large extracellular N-terminal domain. This may provide good accessibility in native and denaturing conditions.
• For western blot, the predicted molecular weight is 93 kDa; an appropriate gel percentage (e.g., 8-10% SDS-PAGE) may be considered.
• Immunofluorescence/immunocytochemistry may require optimization for membrane protein detection; consider using non-permeabilizing conditions for surface staining or permeabilization for total staining.
• The antibody cross-reacts with human, mouse, and rat, making it suitable for multi-species studies; however, endogenous expression levels vary among tissues, so validate in the relevant sample system.
• For ELISA applications, this monoclonal antibody can be used as a capture or detection reagent; pair with a matched antibody for best results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with integrated access to high-quality IVD raw materials. For taste receptor research and related assays, we offer validated monoclonal antibodies like this anti-T1R3/TAS1R3 antibody, along with complementary antibody pairs, bulk reagents, and rare target sourcing. From concept to clinic, our technical services streamline development of immunoassays and biosensors.