Anti-T-bet/Tbx21 Rabbit Recombinant Monoclonal Antibody for WB - Q9UL17

Core Product Specifications and Parameters

Biological Background: T-bet/Tbx21 Function and Localization

• Lineage-defining transcription factor that initiates Th1 lineage development from naive Th precursor cells by activating Th1 genetic programs and repressing the opposing Th2 and Th17 genetic programs. Related references: PMID:10761931
• Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3, and induces permissive chromatin accessibility and CpG methylation in IFNG. Related references: PMID:33296702
• Can activate Th1 genes via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells. Related references: PMID:27292648
• Inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC, and suppresses Th2 cytokines such as IL-4, IL-5, and IL-13 via repression of GATA3 and NFATC2.
• Protects Th1 cells from amplifying aberrant type-I IFN response in an IFN-gamma abundant microenvironment by acting as a repressor of type-I IFN transcription factors and type-I IFN-stimulated genes.
• Localizes predominantly to the nucleus, consistent with its role as a transcription factor.
• Exhibits T-cell specific expression; also required for the correct development of natural killer (NK) and mucosal-associated invariant T (MAIT) cells.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitination, which may regulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 322–535 (C-terminal region) of human T-bet/Tbx21, providing specificity for the full-length protein.
• For Western blot (WB), a band at approximately 58 kDa is expected; verify in a relevant sample such as Th1-polarized human T cells or mouse splenocytes.
• In immunohistochemistry (IHC-P), consider heat-induced epitope retrieval (HIER) in citrate buffer (pH 6.0) for optimal detection.
• For immunoprecipitation (IP), validate antibody performance under native conditions using nuclear extracts from activated T cells.
• Cross-reactivity with mouse and rat T-bet allows inter-species studies; confirm detection in the appropriate tissue or cell line.
• Titrate the antibody by ELISA to determine optimal working concentration for each specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop access to high-quality IVD raw materials, including this anti-T-bet/Tbx21 recombinant monoclonal antibody for immunology and Th1/Th2 differentiation research. As a comprehensive sourcing partner, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to streamline your assay development from concept to clinic. Let us help bridge your procurement needs with reliable, scalable solutions for T-bet-related projects.