Anti-SYT7 Rabbit Polyclonal Antibody for WB and ELISA - O43581

Core Product Specifications and Parameters

Biological Background: Synaptotagmin-7 Function and Localization

• Synaptotagmin-7 (SYT7), also known as IPCA-7 or SytVII, is a calcium sensor protein that belongs to the synaptotagmin family. It contains two C2 domains that bind calcium and phospholipids, mediating Ca²⁺-dependent exocytosis.
• SYT7 plays a critical role in Ca²⁺-triggered lysosomal exocytosis, which is essential for plasma membrane repair following injury. Related references: PMID:11342594
• In the context of infection, SYT7 facilitates cell invasion by Trypanosoma cruzi through Ca²⁺-triggered lysosomal exocytosis. Related references: PMID:11342594 PMID:15811535
• SYT7 is localized to multiple membrane compartments including the cell membrane, presynaptic membrane, lysosome membrane, synaptic vesicle membrane, phagosome membrane, and peroxisome membrane, reflecting its diverse roles in secretion and organelle communication.
• The protein acts as a key mediator of synaptic facilitation, enhancing neurotransmitter release probability by sensing residual Ca²⁺ in presynaptic terminals and promoting synaptic vesicle replenishment.
• SYT7 is widely expressed in a variety of adult and fetal tissues, indicating its broad functional significance in regulated secretion.
• Keywords associated with SYT7 include alternative splicing, calmodulin-binding, lipoprotein, palmitate, and phosphoprotein, highlighting its regulatory complexity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 38-170 near the N-terminus of human SYT7; this region may recognize both full-length and certain splice variants. Consider confirming specificity in your sample system.
• For western blot, prepare samples with reducing conditions and include protease inhibitors, as SYT7 is a membrane-associated protein. The predicted molecular weight is approximately 46 kDa, but post-translational modifications may cause band shifts.
• In ELISA applications, the antibody can be used for direct or indirect detection; optimization of coating antigen concentration and antibody dilution is recommended.
• To confirm cross-reactivity, validate in the relevant human cell lines or tissue lysates known to express SYT7; consider using siRNA knockdown controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, providing validated antibodies like this anti-SYT7 polyclonal antibody for research into calcium-regulated exocytosis and membrane repair. We offer a wide range of monoclonal and polyclonal antibodies, matched antibody pairs, and ancillary reagents to support your assay development and manufacturing needs.