Anti-SYT4 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9H2B2

Core Product Specifications and Parameters

Biological Background: SYT4 Function and Localization

• SYT4 encodes Synaptotagmin-4, a member of the synaptotagmin family that does not bind calcium (by similarity).
• It regulates the mobility of neuronal dense core vesicles (DCVs) through interaction with KIF1A; phosphorylation by MAPK8/JNK1 upon neuronal activation destabilizes this interaction, capturing DCVs at synapses (by similarity).
• SYT4 plays a role in dendrite formation by melanocytes. Related references: PMID:23999003
• The protein is localized to the membrane of cytoplasmic vesicles, particularly neuronal dense core vesicles.
• Expression is detected in melanocytes and in specific brain regions such as the hippocampus, amygdala, and thalamus. Related references: PMID:23999003
• Structurally, SYT4 contains transmembrane regions and is subject to alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 38-190) of human SYT4, which is likely conserved across species, consistent with the observed cross-reactivity with mouse and rat.
• For western blotting, expect a band at approximately 48 kDa. Validate antibody specificity using lysates from cells/tissues with known SYT4 expression.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider optimizing fixation and permeabilization steps, and include controls using brain or melanocyte samples.
• ELISA applications may require titration of antibody and concentration for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to streamline the procurement of high-quality IVD raw materials. This anti-SYT4 antibody supports investigations into neuronal trafficking and melanocyte biology. Complement your workflow with our extensive portfolio of validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents, all backed by efficient sourcing and technical consulting from concept to clinic.