Anti-SYT11 Rabbit Polyclonal Antibody for WB, IF, ELISA - Q9BT88

Core Product Specifications and Parameters

Biological Background: Synaptotagmin-11 Function and Localization

• Synaptotagmin-11 (SYT11), also known as Synaptotagmin XI (SytXI), is a member of the synaptotagmin family involved in vesicular and membrane trafficking. Unlike many synaptotagmins, SYT11 does not bind Ca²⁺.
• SYT11 inhibits clathrin-mediated and bulk endocytosis, ensuring precision in vesicle retrieval, and plays an important role in dopamine transmission by regulating endocytosis and the vesicle-recycling process.
• SYT11 is essential for a neuronal vesicular trafficking pathway distinct from the synaptic vesicle pathway, crucial for development and synaptic plasticity.
• In macrophages and microglia, SYT11 inhibits conventional cytokine secretion (e.g., IL6 and TNF) and phagocytosis, and in astrocytes, it regulates lysosome exocytosis for membrane repair.
• Required for ATP13A2-mediated regulation of the autophagy-lysosome pathway (PubMed:27278822). Related references: PMID:27278822
• Subcellularly, SYT11 localizes to multiple membrane compartments including cytoplasmic vesicle, trans-Golgi network, recycling endosome, lysosome, phagosome, and clathrin-coated vesicle membranes, as well as cell projections like axons and dendrites, and the postsynaptic density.
• Key structural features include two C2 domains and a single transmembrane helix; post-translational modifications include phosphorylation and ubiquitination (by similarity).

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant fragment (aa 37-200) of human SYT11, covering the N-terminal cytoplasmic region. It is suitable for detecting total SYT11 by WB and IF/ICC.
• For Western blot, the predicted molecular weight is approximately 48 kDa; however, the observed band may vary depending on post-translational modifications and cell/tissue type. Include appropriate positive controls (e.g., brain lysates).
• For immunofluorescence and ELISA applications, optimize fixation/permeabilization and antibody dilution in the relevant sample system to confirm specificity.
• Cross-reactivity is reported for human, mouse, and rat; sequence alignment suggests potential detection in other species, but this should be verified experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with integrated sourcing of IVD raw materials, from validated antibodies to bulk ancillary reagents. This SYT11 rabbit polyclonal antibody serves as a reliable tool for neuroscience and autophagy research. Contact us for customized workflows and technical consultation to accelerate your assay development.