Anti-SYT1 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P21579

Core Product Specifications and Parameters

Biological Background: SYT1 Function and Localization

• Synaptotagmin-1 (SYT1) is a calcium sensor that triggers fast neurotransmitter release at synapses by coupling Ca2+ influx to synaptic vesicle exocytosis.
• It is an integral membrane protein of synaptic vesicles, chromaffin granules, and secretory vesicles, with a single N-terminal transmembrane domain and two cytoplasmic C2 domains.
• In addition to its core role in exocytosis, SYT1 regulates synaptic vesicle trafficking and membrane interactions at the active zone through Ca2+-dependent phospholipid binding.
• SYT1 can bind in a Ca2+-independent manner to neurexins, syntaxin, and AP2, linking vesicle docking and endocytosis to the release machinery.
• Plays a role in dendrite formation in melanocytes, as shown by its expression in these cells and functional studies. Related references: PMID:23999003
• The protein is palmitoylated and phosphorylated, modifications that may regulate its membrane association and synaptic function.
• Keywords associated with SYT1 include: Calcium, Synapse, Cytoplasmic vesicle, Lipoprotein, Metal-binding, and Disease variant, highlighting its involvement in neurological processes.
• SYT1 belongs to the synaptotagmin family, which comprises multiple isoforms with distinct expression patterns and Ca2+ sensitivities.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the cytoplasmic region (aa 141-422) encompassing the C2 domains; the antibody is expected to detect total SYT1 protein.
• For Western blot (WB), a band around 48 kDa is anticipated; consider using appropriate loading controls and validate in your cell/tissue lysate model.
• In immunofluorescence/immunocytochemistry (IF/ICC), permeabilization may be required to access the intracellular epitope; confirm staining patterns in neuronal cells.
• For immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized for your tissue type and fixation method.
• This antibody has been tested in ELISA; it is suitable for sandwich or direct detection setups, but pairing with another compatible antibody should be empirically determined.
• Cross-reactivity with mouse and rat SYT1 has been demonstrated, but sequence alignment suggests possible recognition in other species; validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-SYT1 monoclonal antibody is ideal for neuroscience and synaptic research applications, such as studying neurotransmitter release mechanisms. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing, ensuring reliable procurement for your diagnostic or research programs.