Anti-SYPL1 Polyclonal Antibody for IF/ICC, ELISA - Q16563

Core Product Specifications and Parameters

Biological Background: SYPL1 Function and Localization

• SYPL1, also known as synaptophysin-like protein 1 or pantophysin, is a member of the synaptophysin family of integral membrane proteins.
• The human SYPL1 gene encodes a protein that undergoes alternative splicing, producing multiple isoforms.
• SYPL1 is localized to the membrane of cytoplasmic vesicles and has been identified in melanosomes, suggesting a role in intracellular trafficking.
• As a transmembrane protein, SYPL1 contains four transmembrane helices and is glycosylated, typical of proteins involved in vesicle formation and recycling.
• The protein shares structural homology with synaptophysin, a major synaptic vesicle protein, but its exact biological function remains poorly characterized.
• UniProt keywords highlight its presence in cytoplasmic vesicles, its glycoprotein nature, and its role as a reference proteome protein.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 50-120 of human SYPL1, which is an intracellular loop region between transmembrane domains; consider its accessibility in native versus denatured conditions for optimal antibody binding.
• For immunofluorescence (IF/ICC), permeabilization protocols may be necessary as the target epitope is intracellular; validate staining specificity with appropriate controls, such as siRNA knockdown or knockout cell lines.
• In ELISA applications, use a recombinant SYPL1 protein or peptide as a positive control to assess antibody specificity and sensitivity, and optimize coating conditions to preserve epitope conformation.
• As SYPL1 is a membrane protein, sample preparation for Western blot (if attempted) should include mild detergents to maintain solubility and avoid aggregation; however, this application is not specified, so pilot testing is advised.
• Cross-reactivity has been confirmed with human SYPL1; for other species, sequence alignment of the immunogen region may help predict potential reactivity, but experimental validation is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD manufacturers and researchers, offering comprehensive access to raw materials including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For SYPL1 research, we provide high-quality polyclonal antibodies like this one, supporting studies in membrane trafficking and melanosome biology, with the flexibility to source rare targets and customize supply chains to meet your diagnostic development needs.