Anti-Syntrophin alpha 1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q13424

Core Product Specifications and Parameters

Biological Background: Syntrophin alpha 1 Function and Localization

• Alpha-1-syntrophin (SNTA1/SNT1) is an adapter protein that organizes the subcellular localization of various membrane proteins, linking receptors to the actin cytoskeleton and the extracellular matrix via the dystrophin glycoprotein complex.
• It plays a critical role in neuromuscular synapse formation by organizing utrophin (UTRN) and acetylcholine receptors at the postsynaptic membrane.
• The protein binds to phosphatidylinositol 4,5-bisphosphate, which is thought to regulate its membrane association and scaffolding functions.
• Subcellularly, alpha-1-syntrophin localizes to the cell membrane, sarcolemma, cell junctions, and the cortical cytoskeleton.
• Tissue expression is highest in skeletal muscle and heart, with lower levels detected in brain, pancreas, liver, kidney, and lung; it is not detected in placenta.
• The calculated molecular mass is approximately 54 kDa (53.9 kDa), and the protein contains conserved domains characteristic of the syntrophin family.
• Key functional motifs include actin-binding, calcium/calmodulin-binding, and phosphorylation sites; mutations are associated with Long QT syndrome (LQT12).

Experimental Guidance and Technical Tips

• The immunogen (aa 1-90) corresponds to the N-terminal region, which includes a PDZ domain critical for protein-protein interactions; consider this when assessing isoform specificity and potential cross-reactivity.
• For Western blotting, validate detection of endogenous alpha-1-syntrophin in relevant cell lines or tissue lysates, especially those with high expression such as skeletal muscle or heart.
• In immunofluorescence/immunocytochemistry, expect membrane and submembrane cytoskeletal staining; confirm localization patterns in positive control cells.
• ELISA applications may be suitable for quantifying syntrophin levels in biological samples; consider using recombinant protein standards for quantitative assays.
• Always titrate the antibody and include appropriate negative controls to ensure specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop supplier of IVD raw materials, supporting diagnostic developers from concept to clinic. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing for projects involving dystrophin-associated proteins and neuromuscular disease markers. Contact us for bulk orders and custom sourcing solutions.