Anti-Syntenin-1 (Syntenin) Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O00560

Core Product Specifications and Parameters

Biological Background: Syntenin-1 Function and Localization

• Syntenin-1 (also known as MDA-9, TACIP18, SDCBP) is a multifunctional adapter protein involved in diverse cellular processes, including trafficking of transmembrane proteins, neuro/immunomodulation, exosome biogenesis, and tumorigenesis. Related references: PMID:26291527

• It positively regulates TGFB1-mediated SMAD2/3 activation and TGFB1-induced epithelial-to-mesenchymal transition (EMT) and cell migration by enhancing cell-surface expression of TGFR1 via preventing its CAV1-dependent internalization and degradation. Related references: PMID:25893292

• Together with SDC1/4 and PDCD6IP, syntenin-1 plays a key role in exosome biogenesis. Related references: PMID:22660413

• Syntenin-1 regulates migration, growth, proliferation, and cell cycle progression in various cancer types, contributing to its tumorigenic properties. Related references: PMID:26539120

• In adherens junctions, it may couple syndecans to cytoskeletal proteins or signaling components, and it can link the transcription factor SOX4 to the IL-5 receptor (IL5RA). Related references: PMID:11498591

• Subcellularly, syntenin-1 localizes to cell junctions (focal adhesion, adherens junction), cell membrane, endoplasmic reticulum membrane, nucleus, melanosome, cytosol, cytoskeleton, and is also found in secreted extracellular exosomes and membrane rafts.

• At the protein level, it is expressed in lung cancers, including adenocarcinoma, squamous cell carcinoma, and small-cell carcinoma; widely expressed in fetal and adult tissues, with highest levels in heart and placenta. Related references: PMID:25893292

• Post-translational modifications include phosphorylation and acetylation; the protein features lipid-binding domains and is a known phosphoprotein in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human Syntenin-1. For Western blot (WB), consider optimizing antibody dilution based on your sample’s endogenous expression levels and detection system; the predicted molecular weight is ~32kDa.
• For immunohistochemistry (IHC-P), antigen retrieval protocols may be necessary. Validate with tissues known to express Syntenin-1, such as heart, placenta, or lung cancer specimens.
• Immunofluorescence (IF/ICC) may require fixation and permeabilization conditions suited for the subcellular localization of Syntenin-1; confirm staining patterns against expected distributions (e.g., membrane, cytosolic, or junctional).
• In ELISA, this monoclonal antibody can be used as a capture or detection reagent; ensure compatibility with your assay format and perform cross-validation with relevant standards.
• The antibody has been reported to cross-react with mouse and rat, enabling preclinical studies; verify cross-reactivity in your own model systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, supporting diagnostic manufacturers and research laboratories from early-stage R&D through clinical application. This anti-Syntenin-1 monoclonal antibody is well-suited for investigations into exosome biology, TGF-beta signaling, and cancer progression. Through our one-stop sourcing platform, we offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and specialized raw-material sourcing for rare targets, helping streamline your assay development and procurement needs.