Anti-Syntaxin (STX1A/STX1B/STX2/STX3) Rabbit Monoclonal Antibody for WB, IF, IHC, ELISA - Q16623 / P61266 / P32856 / Q13277

Core Product Specifications and Parameters

Biological Background: Syntaxin Function and Localization

• Syntaxin-1A (STX1A), also known as neuron-specific antigen HPC-1, is a member of the syntaxin family of SNARE proteins critical for vesicular trafficking.
• It forms the core SNARE complex with SNAP25 and VAMP2, mediating calcium-dependent fusion of synaptic vesicles with the plasma membrane for neurotransmitter release. Related references: PMID:26635000
• Syntaxin-1A also regulates acrosomal exocytosis in sperm, highlighting a role in fertilization. Related references: PMID:23091057
• Beyond neurotransmission, it participates in hormone exocytosis, such as insulin and GLP-1 secretion (by similarity).
• Subcellularly, syntaxin-1A localizes to the plasma membrane, synaptic vesicle membranes, and secretory vesicles, and is also found secreted.
• It is highly expressed in embryonic spinal cord and ganglia, adult cerebellum and cerebral cortex, and also present in heart, liver, fat, skeletal muscle, kidney, and brain.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin conjugation, which may regulate its function and interactions.
• Keywords: Alternative splicing, coiled coil, exocytosis, transmembrane helix, and involvement in Williams-Beuren syndrome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human syntaxin (P61266/STX1B). This region shares high homology among syntaxin family members, which likely underpins the reported cross-reactivity with STX1A, STX2, and STX3.
• For Western blot (WB), the predicted molecular weight is ~33 kDa. Optimize protein extraction method and loading controls for the tissue or cell type of interest.
• In immunofluorescence (IF) and immunohistochemistry (IHC), consider membrane permeabilization conditions to access the cytoplasmic domain of syntaxin, and validate with appropriate negative controls.
• The antibody's performance in ELISA suggests potential for quantitative detection applications; however, empirical titration and assay optimization are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic developers and research laboratories, offering a comprehensive portfolio of monoclonal and polyclonal antibodies, validated antibody pairs, and ancillary reagents. For studies focused on synaptic function, neurodegeneration, or exocytosis, this syntaxin antibody serves as a reliable research tool, and our team can also source related SNARE protein reagents or develop custom solutions to support your project from concept to clinic.