Anti-Syntaxin 16 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O14662

Core Product Specifications and Parameters

Biological Background: Syntaxin-16 Function and Localization

• Syntaxin-16, encoded by the STX16 gene, is a member of the syntaxin family of SNARE proteins, essential for intracellular membrane fusion.
• It mediates vesicular transport from late endosomes to the trans-Golgi network, a critical retrograde trafficking pathway.
• The protein localizes primarily to the Golgi apparatus membrane, with some cytoplasmic distribution, consistent with its role in vesicle docking.
• Syntaxin-16 is ubiquitously expressed across human tissues, reflecting its fundamental function in membrane trafficking.
• Post-translational modifications include phosphorylation (as indicated by the keyword phosphoprotein), which may regulate its activity and interactions.
• Structural features include coiled-coil domains, characteristic of SNARE proteins, and a transmembrane helix that anchors it to membranes.
• Alternative splicing generates multiple isoforms, potentially expanding functional diversity in different cellular contexts.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100) of human Syntaxin-16; this conserved region likely contributes to the reported cross-reactivity with mouse and rat.
• For western blotting, expect a band at ~37 kDa (UniProt molecular weight: 37,031 Da; supplier-provided weight: 37 kDa). Consider using reducing conditions and appropriate molecular weight markers.
• In immunohistochemistry (paraffin-embedded sections, IHC-P), optimize antigen retrieval and antibody dilution for each tissue type; validation is reported in human, mouse, and rat.
• ELISA-based detection may be employed; validate coating conditions and standard curves using recombinant protein or cell lysates to ensure assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For targets like Syntaxin-16, involved in intracellular trafficking and SNARE biology, we also offer rare target raw-material sourcing to accelerate your assay development from concept to clinic.