Anti-Syntaphilin (SNPH) Rabbit Polyclonal Antibody for WB, ELISA - O15079

Core Product Specifications and Parameters

Biological Background: SNPH (Syntaphilin) Function and Localization

• Syntaphilin is encoded by the SNPH gene (also known as KIAA0374 and bA314N13.5) and functions as a negative regulator of neurotransmitter release.
• It inhibits SNARE complex formation by binding free syntaxin-1A (STX1A), thereby modulating vesicle docking and fusion at the synapse.
• Syntaphilin is anchored to the membrane via a C‑terminal transmembrane helix and is enriched at synaptic terminals and synaptosomes.
• Expression is brain‑specific, with a strict synaptic localization pattern.
• Phosphorylation at multiple sites suggests activity may be dynamically regulated by intracellular signaling.
• Structural motifs include coiled‑coil domains and a transmembrane anchor, consistent with its role in tethering synaptic vesicles.
• Alternative splicing generates multiple isoforms, which may confer functional diversity.
• The protein migrates at an apparent molecular weight of ~54 kDa on SDS‑PAGE, close to the computed mass of 53.5 kDa.

Experimental Guidance and Technical Tips

• The immunogen (residues 300‑425) targets a region that may be conserved across human, mouse, and rat, consistent with the reported cross‑reactivity.
• For Western blot, a band near 54 kDa is expected; consider using brain tissue lysates or synaptic preparations as positive controls.
• Because syntaphilin is phosphorylated, running samples under reducing and non‑reducing conditions may reveal mobility shifts or oligomeric forms.
• In ELISA, coating with recombinant full‑length or the immunogen fragment can improve sensitivity; direct antigen immobilization is recommended for screening.
• Validate the antibody in the relevant species and tissue context — mouse and rat brain lysates are suitable substitutes where human material is limited.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and neuroscience research programs by supplying validated IVD‑grade antibodies and ancillary raw materials. For synthetic biology and synaptic target studies, we offer polyclonal antibodies like this one alongside complementary reagents — from bulk quantities to specialized rare‑target sourcing — to accelerate assay development. Our integrated approach simplifies procurement so you can focus on moving from concept to clinic.