Anti-SYNPO (Synaptopodin) Polyclonal Antibody for WB, ELISA - Q8N3V7

Core Product Specifications and Parameters

Biological Background: Synaptopodin Function and Localization

• Synaptopodin is an actin-associated protein that modulates actin-based shape and motility, playing critical roles in dendritic spines of neurons and podocyte foot processes in the kidney.
• It is essential for the formation of spine apparatuses in telencephalic dendritic spines, a process linked to synaptic plasticity.
• Encoded by the SYNPO gene (KIAA1029), the protein undergoes alternative splicing and is subject to post-translational modifications including acetylation, phosphorylation, and glycosylation.
• Subcellularly, synaptopodin localizes to the cytoplasm, cytoskeleton, cell junctions (tight junctions), perikaryon, dendritic spines, postsynaptic density, and cytosol, indicating diverse compartment-specific roles.
• Tissue expression is prominent in the cerebral cortex, consistent with its role in synaptic architecture and plasticity.
• The protein belongs to the synaptopodin family and contains domains for actin binding, crucial for its association with the cytoskeleton.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (a.a. 1–260) of human synaptopodin. Confirm that the antibody recognizes your target isoform(s) in the relevant sample system.
• For Western blot, the predicted molecular weight is ~99 kDa; use appropriate gel percentages and consider the protein’s potential post-translational modifications that may alter migration.
• Cross-reactivity with mouse and rat is reported, making this antibody suitable for rodent model studies. Validate in brain and kidney tissue lysates.
• For ELISA applications, optimize coating conditions and antibody dilutions using recombinant synaptopodin or peptide as standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for IVD manufacturing and research. For neuroscience and podocyte biology, our synaptopodin antibody is just one example of the rare-target materials we can source. Contact us to streamline your procurement from concept to clinic.