Anti-SYNJ1 Polyclonal Antibody for WB and ELISA - O43426

Core Product Specifications and Parameters

Biological Background: SYNJ1 Function and Localization

• SYNJ1, also known as Synaptojanin-1, is a phosphoinositide phosphatase that hydrolyzes phosphate groups from phosphoinositides and inositol phosphates in a domain-specific manner.
  Related references: PMID:10224048 PMID:18093523 PMID:23804563
• The 5-phosphatase domain catalyzes the removal of the 5-phosphate from substrates such as PtdIns(4,5)P2, PtdIns(3,4,5)P3, Ins(1,4,5)P3, and Ins(1,3,4,5)P4.
  Related references: PMID:18093523 PMID:23804563 PMID:27435091
• The SAC domain hydrolyzes phosphates at the 3- and 4-positions of the inositol ring, targeting PI(3)P, PI(4)P, and PI(3,5)P2.
  Related references: PMID:10224048 PMID:18093523 PMID:23804563
• SYNJ1 localizes to the cytoplasm, perinuclear region, presynapse, and membrane.
• It plays a critical role in synaptic vesicle recycling by participating in ultrafast endocytosis and clathrin uncoating, thereby regulating neurotransmitter release and synaptic plasticity.
• Mutations in SYNJ1 are associated with neurological disorders, including early-onset epileptic encephalopathy (DEE53, EIEE53) and autosomal recessive Parkinson disease (PARK20).
• Post-translational modifications include phosphorylation and methylation, and the protein contains domains for RNA binding.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100–200 of human SYNJ1, an N-terminal region potentially conserved across isoforms. Consider validating antibody specificity with peptide competition assays or recombinant fragment controls.
• For Western blotting under reduced-denaturing conditions, anticipate a band at ~173 kDa; to account for large protein size, optimize transfer times and use a low-percentage polyacrylamide gel for improved resolution.
• In ELISA applications, evaluate binding affinity and specificity against full-length recombinant SYNJ1 protein or the immunogen peptide, and titrate antibody concentrations for optimal signal-to-noise ratios.
• Given cross-reactivity with rat, ensure careful species-matched control lysates or recombinant proteins are used to confirm detection in rodent models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop sourcing partner for IVD raw materials, supporting diagnostic developers from early R&D through clinical application. For neuroscience and neurodegenerative disease research, we offer a comprehensive portfolio including validated antibody pairs, polyclonal antibodies like this anti-SYNJ1 antibody, and bulk ancillary reagents to streamline assay development and scale-up.