Anti-SynGAP Rabbit Monoclonal Antibody for WB and ELISA - Q96PV0

Core Product Specifications and Parameters

Biological Background: SynGAP Function and Localization

• SynGAP (Synaptic Ras GTPase-activating protein 1), also known as Neuronal RasGAP or SYNGAP1, is a 148 kDa multidomain protein encoded by the SYNGAP1 gene (KIAA1938) in humans.
• It serves as a major constituent of the postsynaptic density (PSD) and functions as an inhibitory regulator of the Ras-cAMP pathway, exhibiting dual GTPase-activating protein (GAP) activity toward both Ras and Rap small GTPases.
• As a core component of the NMDAR signaling complex at excitatory synapses, SynGAP contributes to NMDAR-dependent regulation of AMPAR potentiation, membrane trafficking, and synaptic plasticity; it also modulates AMPAR-mediated miniature excitatory postsynaptic currents.
• SynGAP plays a critical role in synaptic plasticity mechanisms that underlie learning and memory, and it may be involved in certain forms of brain injury leading to long-term cognitive deficits.
• Alternative splicing generates multiple isoforms of SynGAP, and the protein is subject to phosphorylation, as indicated by its classification as a phosphoprotein.
• Mutations in SYNGAP1 are associated with neurodevelopmental disorders, including autism spectrum disorder, intellectual disability, and a subtype of autism (MRD5); the protein contains SH3-binding motifs that mediate interactions with synaptic scaffolding proteins.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 1244–1343 of human SynGAP (UniProt Q96PV0), a region conserved in mouse and rat, which likely contributes to the reported cross-species reactivity.
• For Western blot analysis, anticipate a band at approximately 148 kDa; use fresh brain tissue or neuronal cell lysates as positive material and consider including phosphatase inhibitors to preserve phosphorylation status.
• In ELISA applications, the antibody can detect SynGAP in purified or complex samples; optimize coating conditions and detection formats (direct or indirect) for your assay system.
• Given SynGAP’s role in synaptic function, appropriate biological controls (e.g., knockout or knockdown samples, or comparative tissue panels) are recommended to confirm antibody specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers reliable access to this SynGAP rabbit monoclonal antibody and complementary IVD raw materials for neuroscience and synaptic research. From validated antibody pairs and optimized recombinant proteins to bulk ancillary reagents, CamelBio streamlines sourcing for diagnostic and life science manufacturers. For rare or custom targets, our global network supports flexible procurement and technical consultation, helping you accelerate development from concept to clinic.