Anti-SYNE3 Polyclonal Antibody for WB, IF/ICC, ELISA - Q6ZMZ3

Core Product Specifications and Parameters

Biological Background: Nesprin-3 Function and Localization

• Nesprin-3 (SYNE3) is a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, mediating the connection between the nuclear lamina and the cytoskeleton.
• It plays a key role in transmitting mechanical forces across the nuclear envelope and is involved in nuclear movement and positioning.
• Functions as an anchoring protein that tethers the nucleus to the cytoskeleton by binding plectin (PLEC), which can associate with the intermediate filament system.
• Contributes to the regulation of aortic endothelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.
• Localizes to the nucleus outer membrane, nuclear envelope, and rough endoplasmic reticulum.
• Tissue specificity: Expressed in aortic endothelial cells at the protein level.
• The protein contains spectrin repeats and a C-terminal KASH domain, and is subject to alternative splicing.
• Relevant keywords: 3D-structure, Alternative splicing, Coiled coil, Disulfide bond, Endoplasmic reticulum, Membrane, Nucleus, Proteomics identification, Reference proteome, Repeat, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 740-920 of human SYNE3 (NP_689805.3), targeting a region within the central spectrin repeat domain.
• Consider that the polyclonal nature may recognize multiple isoforms and potentially reveal additional bands; validate specificity using relevant controls.
• For WB, use whole cell lysates from human aortic endothelial cells or mouse tissues known to express SYNE3.
• In IF/ICC, ensure proper permeabilization to access the nuclear envelope; co-staining with a nuclear pore marker may aid localization.
• Cross-reactivity with mouse allows for use in mouse models; verify expression levels in the specific tissue or cell type.
• ELISA applications may require optimization of coating antigen and antibody concentration.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized IVD raw-material partner, CamelBio provides diagnostic manufacturers and research labs with reliable access to products like anti-SYNE3 antibodies, supporting studies on nuclear mechanics and the LINC complex. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, complemented by rare target sourcing capabilities. With a B2B-commercial focus, we help streamline your procurement from concept to clinic.