Anti-Syndecan 3 Rabbit Monoclonal Antibody for WB, ELISA - O75056

Core Product Specifications and Parameters

Biological Background: Syndecan-3 Function and Localization

• Syndecan-3 (SDC3), also known as SYND3 or SDCN, is a member of the syndecan family of transmembrane heparan sulfate proteoglycans.
• It functions as a cell surface proteoglycan that may bear heparan sulfate chains, mediating interactions with extracellular matrix components and growth factors.
• Syndecan-3 is implicated in the organization of cell shape by affecting the actin cytoskeleton, possibly through sugar-dependent signal transduction from the cell surface.
• The protein is localized to the cell membrane, consistent with its role as a transmembrane receptor.
• Tissue expression of syndecan-3 is enriched in the nervous system, adrenal gland, and spleen, suggesting roles in neural development and tissue homeostasis.
• Post-translational modifications include the addition of heparan sulfate glycosaminoglycan chains and phosphorylation, which regulate its function.
• The UniProt-reviewed molecular weight of the unmodified protein is approximately 45.5 kDa, with the mature glycoprotein migrating at ~45 kDa in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen for this monoclonal antibody is a synthetic peptide spanning amino acids 1–100 of human syndecan-3 (extracellular domain); therefore, the antibody is expected to recognize the N-terminal region.
• For Western blotting, consider using tissue lysates from brain, adrenal gland, or spleen as positive controls, given the tissue-specific expression pattern.
• Validate the antibody’s cross-reactivity with mouse and rat samples, as indicated by the manufacturer, in your specific experimental system.
• In ELISA, consider coating plates with recombinant syndecan-3 protein or the immunogenic peptide, and titrate the antibody to determine optimal working concentration.
• The predicted molecular weight is ~45 kDa; ensure appropriate molecular weight markers and gel percentage for clear resolution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to provide a seamless supply of IVD raw materials. For targets like syndecan-3, involved in cell surface proteoglycan biology and cytoskeletal organization, we offer validated rabbit monoclonal antibodies, optimized antibody pairs, bulk ancillary reagents, and rare target sourcing. Whether you are developing assays for neuroscience or cell signaling research, CamelBio bridges the gap from concept to clinic with reliable procurement solutions.