Anti-Synaptotagmin XI (SYT11) Rabbit Monoclonal Antibody for WB, ELISA - Q9BT88

Core Product Specifications and Parameters

Biological Background: Synaptotagmin-11 Function and Localization

• Synaptotagmin-11 (SytXI) is a member of the synaptotagmin family of vesicle-trafficking proteins; unlike many family members, it does not bind Ca2+ and is calcium-independent in its functions.
• It acts as an inhibitor of clathrin-mediated and bulk endocytosis, helping to ensure precision in vesicle retrieval, and is critical for dopamine transmission by regulating endocytosis and synaptic vesicle recycling.
• SytXI is an essential component of a neuronal vesicular trafficking pathway distinct from the canonical synaptic vesicle pathway, playing a key role in neuronal development and synaptic plasticity.
• In non-neuronal cells, it inhibits conventional cytokine secretion (e.g., IL6 and TNF) and phagocytosis in macrophages and microglia, while in astrocytes it regulates lysosome exocytosis for plasma membrane repair.
• Required for the ATP13A2-mediated regulation of the autophagy-lysosome pathway, linking it to lysosomal function and cellular clearance mechanisms.
  Related references: PMID:27278822
• Subcellularly, SYT11 localizes to the membranes of multiple organelles and vesicles, including the trans-Golgi network, recycling endosomes, lysosomes, phagosomes, and clathrin-coated vesicles; it is also found in axons, dendrites, and the postsynaptic density.
• Post-translational modifications include phosphorylation and ubiquitination (Ubl conjugation), and the protein spans the membrane with a transmembrane helix.

Experimental Guidance and Technical Tips

• The antibody was generated using a recombinant immunogen covering amino acids 37–200 of human SYT11, an N-terminal region that is likely to detect the full-length protein.
• For Western blotting, a band around 48 kDa is expected; validate in relevant cell lysates from human, mouse, or rat tissue, as cross-reactivity is reported in these species.
• In ELISA applications, optimize coating conditions and include appropriate controls, as the antibody’s performance may vary based on antigen presentation.
• As a rabbit monoclonal antibody, it offers high specificity and batch-to-batch reproducibility; consider it for assays requiring consistent long-term supply.
• Always validate in your specific sample system, and pair with suitable secondary detection reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined channel to access premium IVD raw materials, technical services, and expert consultation from concept to clinical application. As a sourcing partner for the synaptotagmin and vesicle trafficking field, we enable access to validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and sourcing for rare targets like SYT11. Whatever your IVD development needs—whether customized antibody generation or reliable supply—CamelBio bridges the gap to help accelerate your project.