Anti-Synaptotagmin-1 (SYT1) Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P21579

Core Product Specifications and Parameters

Biological Background: Synaptotagmin-1 Function and Localization

• Synaptotagmin-1 (SYT1), also known as p65 or SytI, is a key calcium sensor in the presynaptic terminal that triggers rapid neurotransmitter release from synaptic vesicles.
• It binds acidic phospholipids in a Ca²⁺-dependent manner, facilitating synaptic vesicle docking and fusion at the active zone.
• SYT1 interacts with multiple presynaptic proteins, including neurexins, syntaxin, and AP2, in a Ca²⁺-independent fashion, linking it to vesicle trafficking and endocytosis.
• The protein plays a role in dendrite formation by melanocytes, as shown by functional studies. Related references: PMID:23999003
• Subcellularly, SYT1 is anchored to the membranes of synaptic vesicles, secretory vesicles, and chromaffin granules via a single transmembrane domain.
• Post-translational modifications include glycosylation, palmitoylation (lipoprotein), and phosphorylation, which may regulate its activity and localization.
• Consistent with its membrane association and calcium binding, SYT1 is classified under keywords such as calcium, metal-binding, synapse, and transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the cytoplasmic domain (aa 84-157) of human SYT1, suggesting the antibody may detect both full-length and soluble fragments.
• For Western blotting, the predicted molecular weight is approximately 48 kDa; expect a major band around this size in synaptic protein extracts from human, mouse, or rat brain.
• In immunofluorescence and immunohistochemistry, punctate staining patterns consistent with synaptic vesicle localization should be anticipated; validate with appropriate tissue/cell controls and counterstaining.
• For ELISA, consider using the antibody as a capture or detection reagent after optimizing against recombinant SYT1 protein or native samples.
• Cross-reactivity with other synaptotagmin family members is possible; if needed, verify specificity with isoform-specific controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, core facilities, and researchers with a streamlined, one-stop supply chain for premium IVD raw materials. Complementing this SYT1 polyclonal antibody, our portfolio includes validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents tailored for neuroscience and synaptic vesicle research. Should your project require rare targets or custom-scale sourcing, our team offers tailored support from initial concept to clinical application.