Anti-Synaptotagmin-1 (SYT1) Rabbit Polyclonal Antibody for WB, ELISA - P21579

Core Product Specifications and Parameters

Biological Background: SYT1 Function and Localization

• Synaptotagmin-1 (SYT1), also known as SytI or p65, is a synaptic vesicle protein encoded by the SYT1 gene in humans.
• Acts as a calcium sensor triggering fast neurotransmitter release at synapses; binds acidic phospholipids in a Ca²⁺-dependent manner through its C2 domains.
• Interacts with SNARE proteins (syntaxin), neurexins, and AP2 in a Ca²⁺-independent manner, regulating vesicle docking and endocytosis.
• Plays a role in dendrite formation by melanocytes, as shown in cell-based studies. Related references: PMID:23999003
• Localized primarily to synaptic vesicle membranes, chromaffin granule membranes, and cytoplasmic regions of presynaptic terminals.
• Expressed in brain and melanocytes; belongs to the synaptotagmin family, characterized by an N-terminal transmembrane region and two cytoplasmic C2 domains.
• Post‑translational modifications include glycosylation, palmitoylation, and phosphorylation, which modulate its membrane association and function.
• Mutations in SYT1 have been linked to neurological disorders, underscoring its essential role in synaptic transmission (UniProt Keywords: Disease variant).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (aa 1‑100), enabling detection of full‑length SYT1 and potentially any isoforms sharing this epitope.
• For Western blot, consider using brain tissue lysate (human or mouse) as a positive control; validate the antibody in your specific sample system to confirm the expected 47 kDa band.
• In ELISA, use the synthetic peptide immunogen as an antigen control to assess antibody specificity and titer.
• Cross‑reactivity with mouse has been experimentally confirmed; verify performance in other species/application combinations as required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti‑SYT1 polyclonal antibody as part of a comprehensive portfolio for neuroscience and synaptic research. We provide validated antibody pairs, recombinant antibodies, and bulk reagents, along with expertise in sourcing rare‑target raw materials. From initial concept through clinical application, CamelBio supports diagnostic manufacturers and laboratories with tailored IVD raw material solutions.