Anti-Synaptophysin Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P08247

Core Product Specifications and Parameters

Biological Background: Synaptophysin Function and Localization

• Synaptophysin (SYP), also known as Major synaptic vesicle protein p38, is a transmembrane glycoprotein predominantly expressed in synaptic vesicles of the central and peripheral nervous system.
• It is hypothesized to participate in the structural organization of synaptic vesicle membranes and may facilitate vesicle docking or fusion with the plasma membrane during neurotransmitter release.
• Functional studies indicate its involvement in the modulation of both short-term and long-term synaptic plasticity, though the precise molecular mechanisms remain to be fully elucidated.
• Subcellularly, synaptophysin localizes to the membrane of cytoplasmic secretory vesicles, specifically synaptic vesicles, and is enriched at synapses and synaptosome preparations.
• Tissue specificity analysis reveals high expression in the brain, particularly within the hippocampus, dentate gyrus (outer molecular layer), CA4/CA3 neuropil, and putamen.
• The protein undergoes several post-translational modifications, including glycosylation and phosphorylation, and is calcium-binding. Variants in SYP are associated with intellectual disability (XLID), underscoring its role in neuronal function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide; consider using peptide blocking controls to confirm antibody specificity in your assays.
• For Western blotting (WB), the expected band is approximately 34 kDa. Ensure proper protein extraction from neuronal tissues or synaptic vesicle preparations.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), fix and permeabilize tissue sections or cells appropriately to expose the synaptic vesicle membrane epitope. Validate staining patterns on brain tissue or cultured neurons.
• This antibody demonstrates cross-reactivity with human, mouse, and rat samples, making it suitable for comparative neuroscience studies across these species.
• For ELISA, optimize coating conditions and antibody dilutions empirically, as no validated protocol is provided.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw materials, providing access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. The Synaptophysin Rabbit mAb supports neuroscience research and diagnostic assay development, and we can assist with rare target sourcing, technical consultation, and supply chain solutions to accelerate your project from concept to clinic.