Anti-Synaptophysin Polyclonal Antibody for WB, IP, IF, IHC - P08247

Core Product Specifications and Parameters

Biological Background: Synaptophysin Function and Localization

• Synaptophysin, also known as major synaptic vesicle protein p38, is a 33845 Da transmembrane glycoprotein encoded by the SYP gene.
• It is primarily localized to the membrane of synaptic vesicles and is a key component of synaptosomes, suggesting roles in vesicle organization and trafficking.
• Functionally, it may organize membrane components and target vesicles to the plasma membrane, and is implicated in the regulation of both short-term and long-term synaptic plasticity (By similarity).
• The protein contains four transmembrane helices, undergoes calcium binding, phosphorylation, and ubiquitination, and features alternative splicing leading to multiple isoforms.
• It is highly expressed in brain regions including the hippocampus, dentate gyrus, and putamen, as shown by protein-level detection. Related references: PMID:8838578 PMID:17296554
• Mutations in SYP are associated with X-linked intellectual disability (MRX96), highlighting its critical role in cognitive function.
• It is classified as a reference proteome member and has been identified in proteomics studies, reflecting its abundance in synaptic preparations.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 222-313 of human Synaptophysin, a region predicted to face the cytoplasmic side; thus, the antibody may recognize both denatured and native forms, supporting applications like WB, IP, and IF/ICC.
• Given the high conservation across human, mouse, and rat, cross-reactivity is expected, but validation in the specific experimental system is recommended.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval optimization may be necessary due to the transmembrane nature of the target; test with positive control brain tissue.
• Endogenous Synaptophysin is a 34 kDa protein; a band of this size in WB confirms specificity, and pre-adsorption with the immunogen can serve as a negative control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutions to provide validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents for neuroscience and synaptic biology research. With this anti-Synaptophysin polyclonal antibody and our extensive raw-material catalog, we help you accelerate assay development from concept to clinic.