Anti-Synapsin-1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P17600

Core Product Specifications and Parameters

Biological Background: Synapsin-1 Function and Localization

• Synapsin-1, also designated Brain protein 4.1, is encoded by the SYN1 gene; alternative splicing produces isoforms SYN1a and SYN1b.
• It is a neuronal phosphoprotein that coats synaptic vesicles and binds the cytoskeleton, acting as a key regulator of synaptic vesicle trafficking and neurotransmitter release at the presynaptic terminal. Related references: PMID:21441247 | PMID:23406870
• Synapsin-1 also participates in the regulation of axon outgrowth and synaptogenesis.
• It forms a complex with NOS1 and CAPON that is necessary for specific nitric oxide functions at a presynaptic level.
• Subcellular localization includes synapses, the Golgi apparatus, presynapses, and cytoplasmic secretory vesicles, particularly synaptic vesicles.
• Post-translationally, Synapsin-1 is subject to phosphorylation and methylation; it contains actin-binding domains and is classified as a glycoprotein and phosphoprotein.
• Disease-associated variants of SYN1 are linked to epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence in the central region of human Synapsin-1 (amino acids 401–500). For Western blotting, expect a band at approximately 74 kDa in human, mouse, and rat brain lysates.
• For immunofluorescence and immunohistochemistry, consider using brain tissue sections where Synapsin-1 is highly expressed in neurons; optimize fixation and permeabilization for synaptic marker detection.
• Validate the antibody in your specific experimental system by including appropriate positive and negative controls, and confirm reactivity with your target species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to a complete portfolio of IVD raw materials. For synaptic and neuroscience research, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support in sourcing rare-target raw materials. Whether you need this Synapsin-1 monoclonal antibody or tailored solutions, CamelBio bridges your sourcing requirements from concept to clinic.