Anti-SYK Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P43405

Core Product Specifications and Parameters

Biological Background: SYK Function and Localization

• Non-receptor tyrosine kinase mediating signal transduction downstream of various transmembrane receptors, including B-cell and T-cell receptors, regulating innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation, and vascular development. Related references: PMID:12387735 PMID:33782605

• Assembles into signaling complexes at the plasma membrane via interaction between its tandem SH2 domains and receptor tyrosine-phosphorylated ITAM domains; signaling can also occur through ITAM-independent mechanisms.

• Directly phosphorylates downstream effectors such as VAV1, PLCG1, PI3K, LCP2, and BLNK, initiating key signaling cascades. Related references: PMID:12456653 PMID:15388330 PMID:34634301

• Essential for B-cell development, particularly at the pro-B to pre-B transition, and also functions in T-cell receptor signaling. Related references: PMID:12456653

• Plays a crucial role in innate immunity against fungal, bacterial, and viral pathogens by activating ROS production, inflammasome, and NF-κB-mediated cytokine transcription.

• Required for IL15-stimulated neutrophil phagocytosis and regulates neutrophil degranulation and phagocytosis via MAPK signaling. Related references: PMID:15123770

• Exhibits cell membrane, cytoplasmic, and cytosolic localization; widely expressed in hematopoietic cells including B-cells and neutrophils.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 250-350 of human SYK (NP_001128524.1), a region that is highly conserved across human, mouse, and rat, which may explain the observed cross-reactivity.
• For Western blot analysis (WB), a band around 72 kDa is expected; consider using reducing conditions and whole-cell lysates from B-lymphocyte or hematopoietic cell lines.
• In immunofluorescence (IF/ICC), SYK is anticipated to localize to the cell membrane and cytoplasm; permeabilization is required for intracellular detection.
• ELISA can be performed for quantitative detection; optimization of coating antigen and antibody concentration is recommended for the specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies high-quality antibodies like this anti-SYK polyclonal antibody to support research into B-cell receptor signaling, immune disorders, and hematological diseases. As a dedicated IVD raw material provider, we offer validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents for diagnostic kit development and translational research. Our one-stop sourcing model streamlines access to rare and standard targets, accelerating your path from concept to clinic.