Anti-Syk Monoclonal Antibody for WB, IP, IF, FC, ELISA - P43405

Core Product Specifications and Parameters

Biological Background: Syk Function and Localization

• SYK is a non-receptor tyrosine kinase that regulates innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation, and vascular development. Related references: PMID:12387735, PMID:33782605
• Essential for B-cell maturation, particularly at the pro-B to pre-B transition, and phosphorylates key adapters like BLNK and BTK upon BCR engagement. Related references: PMID:12456653
• Phosphorylates direct downstream effectors such as VAV1, PLCG1, PI-3-kinase, LCP2, and BLNK to propagate immune signaling. Related references: PMID:12456653, PMID:15388330, PMID:34634301
• Plays a crucial role in innate immunity, mediating responses to fungal, bacterial, and viral pathogens via receptors like CLEC7A and activating the inflammasome and NF-κB pathway.
• Required for integrin-mediated cell adhesion and platelet activation, relaying signals from receptors such as ITGB3 engaged by fibrinogen. Related references: PMID:12387735
• Widely expressed in hematopoietic cells, including neutrophils and throughout B-cell development (from pro-B to plasma cells); localizes to the cell membrane and cytoplasm. Related references: PMID:8163536
• Contains two SH2 domains, an ATP-binding kinase domain; keywords underscore roles in angiogenesis and host-virus interaction.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 251-350 (internal region), so the antibody is expected to detect total SYK regardless of phosphorylation status.
• For Western blot, expect a band around 72 kDa; consider using B-cell lines or whole spleen lysate as positive controls. Cross-reactivity with mouse and rat enables multi-species studies.
• In immunoprecipitation, pre-clearing lysates may reduce background; optimize elution conditions for your specific experimental setup.
• For immunofluorescence and intracellular flow cytometry, include a permeabilization step; validate fixation/permeabilization protocols to preserve epitope accessibility.
• The antibody is also suitable for ELISA; consider pairing with a SYK detection antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with validated IVD raw materials, offering a comprehensive portfolio that includes monoclonal antibodies like this anti-SYK clone. With robust supply capabilities for antibody pairs, bulk reagents, and rare-target sourcing, we support your entire development workflow—from early-stage research on signal transduction and hematologic malignancies to final immunoassay production. Trust CamelBio as your single procurement partner for high-quality immunoreceptor study tools.