Anti-SYCE1 Polyclonal Antibody for WB, ELISA - Q8N0S2

Core Product Specifications and Parameters

Biological Background: SYCE1 Function and Localization

• Synaptonemal complex central element protein 1 (SYCE1), also known as cancer/testis antigen 76 (CT76), is a major structural component of the synaptonemal complex (SC).
• SYCE1 localizes to the transverse central element of SCs, which are zipper-like protein structures that form between homologous chromosomes during meiotic prophase.
• Incorporation of SYCE1 into the central element requires the presence of synaptonemal complex protein 1 (SYCP1).
• SYCE1 plays a critical role in SC assembly, stabilization, and meiotic recombination, facilitating proper chromosome segregation.
• The protein is encoded by the SYCE1 gene (GeneID: 93426) and undergoes alternative splicing.
• Subcellularly, SYCE1 is found in the nucleus and associates with chromosomes during meiosis.
• Mutations in SYCE1 are associated with premature ovarian failure (POF12) and spermatogenic failure (SPGF15), underscoring its importance in gametogenesis.
• SYCE1 contains coiled-coil domains, which are typical for structural components of the synaptonemal complex.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 132-351 of human SYCE1, a region that may contain conserved epitopes across species, consistent with reported cross-reactivity with mouse and rat.
• For Western blotting, consider using meiotic cell extracts or tissues rich in synaptonemal complexes, and validate antibody specificity with appropriate controls.
• For ELISA, optimize coating antigen concentration and detection conditions to ensure sensitivity and specificity; recombinant SYCE1 fragments may serve as positive controls.
• Since SYCE1 is a meiosis-specific protein, expression levels may be low in somatic cells; induction of meiosis or enrichment of germ cells might be necessary for reliable detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of high-performance IVD raw materials for reproductive health and meiosis research, including validated antibodies like SYCE1 Rabbit pAb. We offer one-stop access to antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets—supporting your diagnostic and research workflows from early-stage R&D to clinical application. Whether you need custom immunogen design, cross-reactivity guidance, or scalable supply, CamelBio bridges the gap between discovery and commercialization.