Anti-SWAP70 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UH65

Core Product Specifications and Parameters

Biological Background: SWAP70 Function and Localization

• SWAP70 (Switch-associated protein 70) is a 68998 Da protein that acts as a phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) for RAC.
• It transduces signals from tyrosine kinase receptors to RAC independently of RAS, mediating membrane ruffling and regulating the actin cytoskeleton.
• The protein localizes to the cytoplasm, cell membrane, nucleus, lamellipodia, and cytoskeleton, consistent with its roles in cell protrusion and migration.
• SWAP70 is predominantly expressed in mature B-cells, and also found in spleen, kidney, lung, liver, monocytes, and macrophages. Related references: PMID:10681448 PMID:12925760
• The protein contains DNA-binding and coiled-coil domains and undergoes phosphorylation, as indicated by UniProt keyword annotations.
• The expected molecular weight is 69kDa (based on NP_055870.2), matching the product's reported weight.

Experimental Guidance and Technical Tips

• The immunogen (aa 316–585) targets the C-terminal region; consider potential cross-reactivity with SWAP family proteins and validate specificity in your system.
• For Western blotting (WB), a band at ~69 kDa is expected; use positive controls such as B-cell lines or spleen tissue lysates.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization, as SWAP70 localizes to multiple subcellular compartments.
• Titrate the antibody for ELISA, and use the recombinant immunogen as a standard to establish reliable detection limits.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic and research laboratories streamlined access to essential IVD raw materials, including this anti-SWAP70 polyclonal antibody for investigations into B-cell biology and signal transduction. Beyond individual antibodies, CamelBio provides validated antibody pairs, optimized monoclonal and polyclonal preparations, bulk ancillary reagents, and support for sourcing rare targets—empowering projects from concept to clinical application.