Anti-SUV39H2 Polyclonal Antibody for WB, ELISA, ChIP - Q9H5I1

Core Product Specifications and Parameters

Biological Background: SUV39H2 Function and Localization

• SUV39H2 is a histone-lysine N-methyltransferase, also known as KMT1B or Suppressor of variegation 3-9 homolog 2, that specifically trimethylates histone H3 at Lys-9 (H3K9me3) using monomethylated H3K9 as substrate. H3K9me3 serves as a docking site for HP1 proteins, leading to heterochromatin formation and gene silencing. Related references: PMID:10949293
• It plays a central role in establishing constitutive heterochromatin at pericentric and telomere regions, where H3K9me3 also directs DNA methylation at pericentric repeats. Related references: PMID:10949293 PMID:41094145
• SUV39H2 interacts with RB1 to regulate cell cycle progression, transcriptional repression, and telomere length homeostasis. Related references: PMID:14765126
• Subcellular localization is nuclear, with enrichment at centromeric chromatin and chromosome structures.
• SUV39H2 is a phosphoprotein that undergoes phosphorylation and possibly ubiquitin-like conjugation, modulating its activity.
• During spermatogenesis, it contributes to higher-order chromatin organization (by similarity).
• It participates in circadian rhythm regulation by being recruited to E-box elements, inducing heterochromatin-like repression of clock genes (by similarity).

Experimental Guidance and Technical Tips

• The immunogen, corresponding to amino acids 141-350 (NP_001180354.1), encompasses the catalytic SET domain; this region is highly conserved, supporting cross-reactivity with mouse and rat.
• For Western blot analysis, a predominant band near 47 kDa is expected; consider optimizing sample preparation and antibody dilution for clear detection in human, mouse, and rat nuclear extracts.
• In ChIP assays, antibody specificity for SUV39H2 should be validated with appropriate cross-linking conditions and controls to enrich H3K9me3-marked heterochromatin loci.
• ELISA may be used to assess antibody titer and binding affinity; consider pairing with a suitable secondary antibody for quantitative detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to IVD raw materials, technical services, and expert consulting for diagnostic developers and research laboratories. For epigenetics and chromatin biology studies, we offer validated anti-SUV39H2 antibodies, optimized polyclonal and monoclonal raw materials, and bulk ancillary reagents to streamline your assay development from concept to clinic. Whether you need customized antibody pairs, rare-target sourcing, or reliable supply chains, CamelBio bridges your research to clinical translation.