Anti-SUV39H1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O43463

Core Product Specifications and Parameters

Biological Background: SUV39H1 Function and Localization

• SUV39H1 is a histone-lysine N-methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3) using monomethylated H3K9 as substrate. It also weakly methylates histone H1 in vitro. Related references: PMID:10949293, PMID:11242053, PMID:18004385

• H3K9me3 serves as a binding platform for HP1 proteins (CBX1, CBX3, CBX5), leading to epigenetic transcriptional repression and heterochromatin formation. Related references: PMID:10949293, PMID:11242053, PMID:18004385

• Functions primarily in constitutive heterochromatin at pericentric and telomere regions, and directs DNA methylation at pericentric repeats. Related references: PMID:41094145

• Involved in diverse cellular processes: repression of MYOD1-stimulated differentiation, regulation of cell cycle exit and differentiation, repression by PML-RARA fusion protein, BMP-induced repression, and regulation of telomere length. Related references: PMID:11484059, PMID:14765126, PMID:16449642

• Component of the eNoSC complex, which mediates energy-dependent silencing of rDNA. Upon glucose starvation, SIRT1 deacetylates H3, followed by SUV39H1-mediated H3K9me2, leading to silent chromatin at rDNA loci. Related references: PMID:18485871

• Plays a role in defense against mycobacterial infections by methylating M.tuberculosis HupB protein, inhibiting bacterial growth. Macrophages with reduced SUV39H1 are more susceptible to infection.

• Subcellular localization includes nucleus, chromosome, nucleoplasm, centromere, nuclear lamina, cytoplasmic vesicle (phagosome lumen), and cell membrane.

• Post-translational modifications include acetylation, phosphorylation, and ubiquitylation. Contains zinc finger domains and is S-adenosyl-L-methionine-dependent.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–220) of human SUV39H1. Consider that this region may be conserved across species, supporting the observed cross-reactivity with mouse and rat.
• This polyclonal antibody is suitable for WB, IHC-P, and ELISA. For optimal results, validate dilution factors and experimental conditions in the relevant sample system.
• SUV39H1 localizes primarily to the nucleus and chromatin; ensure appropriate nuclear extraction or permeabilization protocols for detection in cell-based assays.
• Due to its role in heterochromatin, expression may be low in some cell types; consider enrichment strategies or positive controls (e.g., proliferating cells) to enhance signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams with streamlined access to IVD raw materials. For epigenetic studies targeting SUV39H1, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support your assay development. Our one-stop sourcing also covers rare target raw materials, ensuring you move from concept to clinic efficiently.