Anti-Survivin Rabbit PolymAb® Antibody for WB and ELISA - O15392

Core Product Specifications and Parameters

Biological Background: Survivin Function and Localization

• Survivin (BIRC5), a member of the inhibitor of apoptosis (IAP) family, localizes to centromeres, spindle, and midbody, and promotes cell proliferation while inhibiting apoptosis. Related references: PMID:20627126, PMID:21364656, PMID:25778398

• As a component of the chromosomal passenger complex (CPC), survivin is essential for chromosome alignment and segregation during mitosis and cytokinesis. Related references: PMID:16322459

• Survivin directs CPC localization from the inner centromere in prometaphase to the midbody during cytokinesis and organizes the central spindle by associating with polymerized microtubules. Related references: PMID:20826784

• It recruits CPC to centromeres at early mitosis through interaction with histone H3 phosphorylated at Thr-3 (H3pT3). Related references: PMID:20929775

• A complex with RAN facilitates mitotic spindle formation by serving as a scaffold for TPX2 delivery to microtubules. Related references: PMID:18591255

• Survivin counteracts apoptosis in G2/M phase, inhibits CASP3 and CASP7, and is essential for mitochondrial integrity and function. The acetylated form represses STAT3 transactivation of target gene promoters. Related references: PMID:9859993, PMID:21536684, PMID:25778398, PMID:20826784

• Isoform 2 and isoform 3 are not vital for mitosis; isoform 3 exhibits markedly reduced anti-apoptotic effects compared to the wild-type form. Related references: PMID:12773388, PMID:16291752, PMID:10626797

• Expressed in fetal kidney and liver; abundantly overexpressed in adenocarcinomas (lung, pancreas, colon, breast, prostate) and high-grade lymphomas. Also detected in cochlear structures. Related references: PMID:10626797, PMID:14741722, PMID:16329164, PMID:20627126, PMID:21364656

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length survivin (aa 1–142) sequence, making this antibody suitable for detecting both wild-type survivin and its isoforms. Because the predicted molecular weight is ~16 kDa but the observed band may range 8–18 kDa, consider including positive and negative controls and validating the band pattern in your specific sample system.
• For western blotting, use a reducing agent and boil samples appropriately. A band around 16–18 kDa is expected in lysates from proliferating cancer cells (e.g., HeLa, Jurkat) or fetal tissues, but expression may be low in normal adult tissues.
• For ELISA applications, pairing with a suitable capture or detection antibody that recognizes a different epitope is recommended. Validate the antibody pair using recombinant survivin protein or cell lysates with known expression levels.
• Cross-reactivity with mouse and rat orthologs allows flexible model system use; however, confirm reactivity in your experimental context with appropriate species-specific controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with one-stop access to IVD-grade raw materials tailored to apoptosis and oncology biomarker workflows. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies against rare targets like survivin, and bulk ancillary reagents—all supported by reliable supply chains. Whether you need high-specificity antibodies for western blot or matched pairs for sandwich ELISA, CamelBio simplifies your sourcing from bench to diagnostic kit assembly.