Anti-Survivin Rabbit Polyclonal Antibody for WB, IF/ICC - O15392

Core Product Specifications and Parameters

Biological Background: Survivin Function and Localization

• Survivin (BIRC5/API4) is a 16.4 kDa IAP family protein that acts as a dual regulator of cell division and apoptosis, essential for mitosis and inhibiting caspase-3/7 activity. Related references: PMID:20627126 PMID:21364656 PMID:25778398
• As a core component of the chromosomal passenger complex (CPC), Survivin is critical for proper chromosome alignment, segregation, and cytokinesis. Related references: PMID:16322459
• It dynamically localizes to cytoplasm, nucleus, centromeres, spindle microtubules, and midbody, guiding CPC positioning throughout mitosis. Related references: PMID:20826784
• Survivin binds histone H3 phosphorylated at Thr-3 to recruit CPC to centromeres. Related references: PMID:20929775
• Through complex formation with RAN, Survivin contributes to mitotic spindle assembly by targeting TPX2 to microtubules. Related references: PMID:18591255
• Its anti-apoptotic function involves direct inhibition of caspase-3 and caspase-7, and it may counteract apoptosis in G2/M phase. Related references: PMID:9859993 PMID:21536684
• Survivin is overexpressed in numerous cancers (lung, pancreas, colon, breast, prostate, lymphomas) but is largely absent in normal adult tissues except fetal kidney/liver and some cochlear cells. Related references: PMID:10626797 PMID:14741722 PMID:16329164
• Alternative splicing yields isoforms 2 and 3, which lack strong anti-apoptotic activity and are not essential for mitosis. Related references: PMID:10626797 PMID:12773388 PMID:16291752

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-142 of human Survivin, covering the N-terminal region. This polyclonal antibody may detect full-length Survivin and potentially isoforms 2 and 3, which share portions of this sequence.
• For Western blot (WB), a band near 16 kDa is expected in human and rat lysates. Due to tissue-specific expression, positive controls such as cancer cell lines (e.g., HeLa, A549) are recommended; normal tissue lysates may show weak or no signal.
• For immunofluorescence/immunocytochemistry (IF/ICC), fixation and permeabilization conditions should be optimized. Survivin localizes to multiple mitotic structures; staining may be most prominent in actively dividing cells. Co-staining with mitotic markers (e.g., phospho-histone H3) can help confirm specificity.
• Consider testing a range of antibody dilutions in both applications, and include appropriate positive and negative controls to validate performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated antibodies, antigens, and ancillary reagents for apoptosis and cell division research. We offer this anti-Survivin polyclonal antibody alongside complementary products such as recombinant Survivin protein and matched monoclonal options to streamline assay development. Our end-to-end supply solutions support diagnostic manufacturers and laboratories from concept validation through clinical-scale production.