Anti-Survivin Rabbit Polyclonal Antibody for WB, ELISA - O15392

Core Product Specifications and Parameters

Biological Background: Survivin Function and Localization

• Survivin (BIRC5) is a multitasking protein with dual roles in promoting cell proliferation and preventing apoptosis. Related references: PMID:20627126, PMID:21364656, PMID:25778398
• It is a core component of the chromosomal passenger complex (CPC), essential for chromosome alignment, segregation, and cytokinesis. Related references: PMID:16322459
• Survivin directs CPC localization from inner centromeres to the spindle midzone and midbody, and organizes the central spindle via microtubule association. Related references: PMID:20826784
• It recruits CPC to centromeres by binding phosphorylated histone H3 (Thr-3) during early mitosis. Related references: PMID:20929775
• The protein forms a complex with RAN to scaffold TPX2 onto microtubules, aiding mitotic spindle formation. Related references: PMID:18591255
• As an inhibitor of apoptosis, survivin suppresses caspase-3 and caspase-7 activity. Related references: PMID:21536684
• Acetylated survivin represses STAT3-driven transcription. Related references: PMID:20826784
• Survivin is essential for maintaining mitochondrial integrity and function. Related references: PMID:25778398
• It is predominantly expressed in fetal tissues and overexpressed in various adenocarcinomas and lymphomas, with specific cochlear expression. Related references: PMID:10626797, PMID:14741722, PMID:16329164, PMID:20627126, PMID:21364656
• Isoform 3 (Survivin-ΔEx3) shows reduced anti-apoptotic activity; isoforms 2 and 3 do not play vital roles in mitosis. Related references: PMID:12773388, PMID:16291752, PMID:10626797
• Subcellular localization includes cytoplasm, nucleus, centromeres, kinetochores, spindle microtubules, and midbody.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitination regulate its diverse functions.

Experimental Guidance and Technical Tips

• As a polyclonal antibody generated against the full-length recombinant protein (aa 1–142), it may recognize multiple epitopes and provide robust signal in denaturing Western blots.
• For Western blot, consider testing antibody dilutions between 1:500 and 1:2000 and validate with appropriate positive and negative controls in your sample system. The predicted molecular weight is ~16 kDa; run suitable molecular weight markers.
• For ELISA, the antibody can be used as a capture or detection reagent; perform pairing optimization to establish the best assay conditions.
• Cross-reactivity with mouse and rat is expected based on sequence homology; however, always verify specificity in the relevant species.
• To avoid freeze-thaw damage, aliquot and store at -20°C or below upon receipt.

CamelBio: Your One-Stop Sourcing Bridge

This survivin antibody is one of many apoptosis and cancer research tools available through CamelBio. As a one-stop IVD raw-material partner, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Contact us for rare target sourcing or custom manufacturing needs.