Anti-SURF1 Rabbit Polyclonal Antibody for WB and ELISA - Q15526

Core Product Specifications and Parameters

Biological Background: SURF1 Function and Localization

• SURF1 encodes the surfeit locus protein 1, a mitochondrial inner membrane protein and component of the MITRAC complex.
• It plays a critical role in the assembly of cytochrome c oxidase (Complex IV), the terminal complex of the mitochondrial respiratory chain.
• Mutations in SURF1 cause Leigh syndrome (MC4DN1), a neurodegenerative disorder, and Charcot-Marie-Tooth disease type 4K (CMT4K), underscoring its essential function.
• Alternative splicing produces multiple isoforms, which may have distinct functions or expression patterns.
• The protein contains transmembrane helices, consistent with its integral membrane localization.
• SURF1 is conserved across species, with orthologs in human, mouse, and rat (reflected by antibody cross-reactivity).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 80-275 of human SURF1 (NP_003163.1), a central region likely conserved across isoforms; verify that your target isoform contains this epitope.
• For Western blot, consider using mitochondrial-enriched lysates from human, mouse, or rat cells as positive controls, and optimize antibody concentration to detect the ~33 kDa band.
• In ELISA, the antibody can be used for antigen capture or detection; perform titration experiments to determine optimal working dilution.
• As SURF1 is a membrane protein, ensure sample preparation includes appropriate detergents to maintain protein solubility and epitope availability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials and technical services. This anti-SURF1 rabbit polyclonal antibody is part of our offering for mitochondrial research and assay development. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, recombinant proteins, or bulk ancillary reagents for large-scale production, CamelBio streamlines your sourcing from concept to clinic.