Anti-SUPV3L1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IYB8

Core Product Specifications and Parameters

Biological Background: SUPV3L1 Function and Localization

• ATP-dependent RNA helicase SUPV3L1, also known as suppressor of var1 3-like protein 1 (SUV3-like protein 1). It belongs to the helicase family and contains a transit peptide for mitochondrial import.
• Major helicase player in mitochondrial RNA metabolism. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PubMed:29967381). Related references: PMID:29967381
• Exhibits ATP-dependent multisubstrate helicase activity, able to unwind double-stranded DNA and RNA, and RNA/DNA heteroduplexes in the 5'-to-3' direction.
• Plays a role in the mitochondrial RNA surveillance system, regulating the stability of mature mRNAs, removing aberrantly formed mRNAs, and rapidly degrading non-coding processing intermediates.
• Implicated in recombination and chromatin maintenance pathways. May protect cells from apoptosis and associates with mitochondrial DNA.
• Subcellular localization: found in the nucleus, mitochondrial matrix, and mitochondrial nucleoid.
• Broadly expressed across tissues, as indicated by tissue specificity data.
• Post-translational modifications include acetylation and phosphorylation. Keywords: 3D-structure, Acetylation, ATP-binding, Helicase, Hydrolase, Mitochondrion, Mitochondrion nucleoid, Nucleotide-binding, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Transit peptide.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 577-786) of human SUPV3L1, which may enhance specificity for the target protein. Validate in the relevant sample system to confirm expected reactivity.
• For Western blot (WB), a band around 88 kDa is expected based on the predicted molecular weight. Consider using appropriate loading controls and mitochondrial fractionation if needed.
• In immunofluorescence/immunocytochemistry (IF/ICC), the protein localizes to mitochondria and nucleus; co-staining with organelle markers is recommended to confirm localization.
• The antibody cross-reacts with mouse and rat, enabling studies across these species. However, always verify cross-reactivity in your experimental setup.
• For ELISA applications, consider optimizing antibody concentration and assay conditions for your specific antigen detection needs.
• Given the protein's involvement in mitochondrial RNA metabolism, experimental conditions such as mitochondrial isolation or specific inhibitors may be explored to modulate its activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-SUPV3L1 polyclonal antibody supports research into mitochondrial RNA metabolism, degradosome function, and related disease models. Beyond this product, CamelBio offers an extensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and efficient sourcing assistance for rare or emerging targets, ensuring seamless procurement for your IVD development needs.