Anti-SUPT7L Rabbit Polyclonal Antibody for WB and ELISA - O94864

Core Product Specifications and Parameters

Biological Background: SUPT7L Function and Localization

• SUPT7L encodes the STAGA complex 65 subunit gamma (also known as STAF65gamma, Suppressor of Ty 7-like, and adenocarcinoma antigen ART1).
• As a component of the STAGA transcription coactivator-HAT complex, SUPT7L is involved in transcriptional regulation.
• The protein localizes to the nucleus, consistent with its role in transcription.
• Tissue expression is highest in adenocarcinomas, gliomas, and thymus, with lower levels in esophageal cancers, malignant hematological disease, peripheral blood mononuclear cells, stomach, small intestine, and skeletal muscle.
• Post-translational modifications include phosphorylation and Ubl conjugation (isopeptide bond), and the gene undergoes alternative splicing.
• UniProt keywords indicate associations with congenital generalized lipodystrophy and reference proteome status.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is ~46 kDa; validate the band size in the relevant sample system and consider loading controls appropriate for the target tissue.
• In ELISA applications, optimize antigen coating and antibody dilutions using known positive controls to confirm specificity.
• The immunogen (aa 210-400) suggests the antibody may recognize isoform-specific epitopes; verify reactivity across alternative spliced variants if needed.
• Cross-reactivity with mouse and rat indicates epitope conservation, but we recommend validation in each species’ sample matrix.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; include internal reference standards in critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. For transcription regulation research, we supply high-quality antibodies like this SUPT7L polyclonal antibody, along with bulk ordering and custom sourcing options. Explore our portfolio for rare targets and comprehensive technical support to streamline your diagnostic development from concept to clinic.